PPT-DNA:chromatin

interactions Exploring transcription factor binding and the epigenomic landscape Lisa Stubbs Eukaryotic genomes are complex structures comprised of modified and unmodified DNA RNA and many types of interacting proteins. Exploring transcription factor binding and the . epigenomic. landscape. Saurabh. Sinha. (several slides here are courtesy of . Lisa . Stubbs). Eukaryotic genomes . are. complex structures comprised of modified and unmodified DNA, RNA and many types of interacting proteins. Edited by: Talebi AR. . Ph.D. Sperm chromatin/DNA remodeling. Testicular phase (. histone-protamine. substitution). . Epididymal. phase (disulfide bonds formation). . Ejaculation phase (zinc addition). interactions. Exploring transcription factor binding and the . epigenomic. landscape. Lisa Stubbs. Eukaryotic genomes . are. complex structures comprised of modified and unmodified DNA, RNA and many types of interacting proteins. The bacterial chromosome is a double-stranded, circular DNA molecule associated with a small amount of protein. Eukaryotic chromosomes have linear DNA molecules associated with a large amount of protein. Waddington, 1942. “The study of any potentially stable, and ideally, heritable change in gene expression or cellular phenotype that occurs without changes in Watson-Crick base-pairing of . DNA” . DNA damage DNA gets damaged a lot ! DNA damage DNA gets damaged a lot! >200,000 events/human cell/day DNA damage Occurs 2 ways 1) spontaneously 2) mutagens : damage DNA Transposons Discovered by Barbara McClintock By. Dr. . Walaa. . Bayoumie. El . Gazzar. Chromatin. The simplest form of chromatin is . present . in non dividing eukaryotic cells, when chromosomes are not sufficiently condensed to be visible by light microscope.. Split . genes: Exons and introns. Chromatin structure and . states: Epigenetics. 12/21/14. 1. Classification by +/- nucleus. Prokaryotes: Organisms whose cells lack a nucleus. Bacteria and . Archaea. 3D . genome organisation. Vladimir Teif. BS222 – Genome Science Lecture 6. Module structure. Genomes, sequencing projects and genomic databases (VT. ). Sequencing . technologies (VT. ). What is a gene? . Genome in eukaryotes is large enough to be accommodated in tiny nucleus. It is required to achieve high degree of compaction for getting into the nucleus. Compaction is achieved by folding the DNA in ZOO LOGY Cell Biology Cell Regulatory Mechanisms : Chromatin Remodeling 1 Development Team Paper Coordinat or: Prof. Kuldeep K. Sharma Department of Zoology, University of Jammu Principal Investigat histones. (50%). Five major . histones. ; H2A, H2B, H3, H4 and H1 highly basic (. Arg. and Lys >25%). Roger Kornberg in 1974; . Chromatin. is made up of repeating units called . nucleosomes. , each containing 200 . S. cerevisiae. strains to winemaking conditions by means of directed evolution and competition experiments with bar-coded YKO strains. Long-term objective. Comprehensive i. dentification of genes involved in the adaptation of . . phase. . separation. (LLPS) under . physiologic. . conditions. Linker. DNA . length. and . patterning. , . histone. H1, and . acetylation. modulate . chromatin. LLPS. Acetylated. . chromatin.