PPT-Regulation of Gene Expression

13 February 2013 Ansuman Chattopadhyay PhD Head Molecular Biology Information Services Health Sciences Library System University of Pittsburgh ansumanpittedu Topics retrieve promoter sequences. Inducible gene expression. kinetics of . β-galactosidase. enzyme induction. Add inducer. start transcription = mRNA accumulation. mRNA translation = protein accumulation. Remove inducer. Stop. transcription (. The problem to be solved (an example). Hauf, J., Zimmermann, F.K., M. ü. ller, S., 2000. Simultaneous genomic over expression of seven glycolytic enzymes in the yeast Saccharomyces cerevisiae. Ezyme. Microbiol. Technol.. Understanding:. Gene expression is regulated by proteins that bind to specific base sequences in DNA . The environment of a cell and of an organism has an impact on gene expression. Nucleosomes help to regulate transcription in eukaryotes. Eukaryotic Gene Regulation. How are genes turned on & off . in eukaryotes?. How do cells with the same genes differentiate to perform completely different, specialized functions?. Evolution of gene regulation. What does the operon model attempt to explain?. the coordinated control of gene expression . in bacteria. bacterial resistance to antibiotics. how genes move between homologous regions of DNA. the mechanism of viral attachment to a host cell. . Differential . expression, clustering, networks, and functional enrichment. STEMREM 201 Fall 2012. Aaron . Newman, Ph.D.. 10/17/12. A . genomics approach . to . biology involves…. A plethora of . Outline. Objective. Mechanism. K. inase cascades and Phosphorylation/. dephosphorylation. . General. Learning/LTP. Synaptic strengthening. Control of gene expression. Transcription factors. Epigenetics. CSCOPE. Unit: 08 Lesson: 01. There are hundreds of different types of cells in your body, and each type has a unique function. We’re going to compare some different types of cells to see how much they have in common. . How do we regulate the expression of our genes? . Involved in gene expression. DNA regulatory sequences. Regulatory genes. Small regulatory proteins (. RNAs. ). Regulatory sequences. Stretches of DNA that interact with regulatory proteins to control transcription.. The cerebellum is the region of the brain that coordinates motor control, and many human neural disorders impact the cerebellum.. Several spontaneous mouse mutants with motor defects have been found, including:. Gene Expression. Gene Expression. Transcription. Splicing. Polyadenylation. mRNA Stability. Translation. Protein Stability. Controls on Protein Levels. Transcription Control - Prokaryotic Promoter. Transcription Control - Prokaryotic Promoter. RNA can destabilize mRNA. Single-stranded RNA can affect gene expression. Double-stranded RNA can affect gene expression. All of above. . Before their study, it is known that. Single stranded RNA can cause RNA interference. Regulating . PROKARYOTIC. Gene Expression. Both prokaryotes and eukaryotes . alter their patterns of gene expression . in . response to changes in environmental conditions. .. During development, gene expression must be carefully regulated to ensure that the right genes are expressed only at the correct time and in the correct place.. Systematic . In . silico. . analysis using public database. Sung Hwan Lee. 1,4. , Baek Gil . Kim. 2,3. , . Ho Kyoung Hwang. 1,4. , Woo . Jung . Lee. 1,4. , Chang . Moo Kang. 1,4**.  . 1. Department .