PPT-Additional file 4: Comparison of RNA-

Seq and RTqPCR expression analyses of candidate genes between RIL165 and RIL387 Additional file 4 Comparison of RNA Seq and RTqPCR expression analyses of candidate genes between RIL165 and RIL387. USER GUIDE For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.Information in this document is subject to change without notice.DISCLAIMERSLIFE TECHNOLOGIES CORPO 7. 7 RNA Synthesis and Processing. Chapter Outline. Transcription in Prokaryotes. Eukaryotic RNA Polymerases and General Transcription Factors. Regulation of Transcription in Eukaryotes. RNA Processing and Turnover. Yann. . Ponty. (VARNA), CNRS/. Ecole. . Polytechnique. , France. Jim Procter . (. JalView. ), University of Dundee, UK. Goals. To help your . survive. . the . RNA data jungle. .. To conceptually and . DNA. Replication. Transcription. Translation. Polymerase. Monomers. DNA Pol III . (and I). dNTPs. Direction . of . synthesis. 5. ’. to . 3’. Template. ssDNA. Product. polynucleotide. RNA. Protein. A (soon to be outdated) Tutorial. A Brief History of Sequencing and Gene Expression. Limitations of Sanger Sequencing. Low throughput. Inconsistent base quality. Expensive. Not quantitative. Frederick “. When DNA codes for RNA, the process is called . TRANSCRIPTION. . When RNA codes for proteins, the process is called . TRANSLATION. .. There are 3 types of . rna. , however only mRNA is involved in transcription. H-FILE P-FILE H-FILE P-FILE H-FILE P-FILE TECHNICAL VARIABLES SOCIO-DEMOGRAPHIC VARIABLES irregular/casual/odd jobs for payODDJOB_CIdentifiersLiving arrangementsunofficial/non-registered/untaxed workN RNA species in cells. RNA processing. Types of introns and their removal. Types of introns. Complex organisms have intron-rich genes. Examples of introns in human protein-coding genes. Removal of introns from mRNA in the nucleus of eukaryotes. Stryer. Short course. Chapter 33. Nucleic Acid Structure. Nucleobase. Nucleoside. Nucleotide. Nucleic acid. Chromatin. Chromosome. Polymeric Structure. Polymer ideal for informational molecule. Ribose and deoxyribose. PHANG LAB TALK. MAY 03, 2012. Transcriptome. The collection of . all. transcripts (RNA) presents in a given cell . ~5 % codes for proteins. The rest codes other variety of RNAs. Speculation!. Most of the genome is transcribed into some form of RNAs. Nucleic Acids. What do we know already?. i. . The nucleic acids DNA and RNA are polymers of nucleotides. Nucleotides consist of 3 parts. A 5-carbon sugar – ribose or deoxyribose. A phosphate group (acidic & negatively-charged). COMPARISON OF ADJECTIVES DEGREES OF COMPARISON DEGREES OF COMPARISON COMPARATIVE DEGREE (Grau Comparativo) Compara UM elemento com OUTRO . Nessa comparação poderá haver IGUALDADE, DESIGUALDADE, SUPERIORIDADE Jeremy Buhler. for GEP Alumni Workshop. RNA-Seq Pipeline for Expression Analysis. RNA Source. 37251. 20653. 9827. 5121. RNA-Seq Read Count . per Transcript. Map reads to transcripts. RNA Abundance. nano. -switches to detect novel non-coding RNA expression . .  . Scott . A. . Tenenbaum.  . SUNY- College of Nanoscale Science and Engineering, Nanobioscience. The Basic sxRNA Concept. +. OR. Structurally interacting RNA .