PPT-Studying gene expression: An improved

Studying gene expression An improved highcontent RNAi screening technology Discovering more faster costeffectively Dr Asongwe L A Tantoh Introduction T his presentation is intended to. Inducible gene expression. kinetics of . β-galactosidase. enzyme induction. Add inducer. start transcription = mRNA accumulation. mRNA translation = protein accumulation. Remove inducer. Stop. transcription (. CONSTITUTIVE GENE PRODUCTS ARE NEEDED BY THE BODY AT ALL TIMES. TUMOR SUPRESSOR PROTEINS. ATP. ENZYMES THAT CONTROL CELLULAR RESPIRATION. ADAPTIVE GENE PRODUCTS ARE ONLY REQUIRED UNDER CERTAIN CONDITIONS. Stephen Montgomery. smontgom@stanford.edu. montgomerylab.stanford.edu. @. sbmontgom. Identifying the genes and variants responsible for disease.. Monogenic to polygenic diseases. Rare to common variants. Advances in Gene Expression Science . Allow Scientists to Compare & Contrast . Expression Patterns of two Biological States. Biological State #1. Biological State #2 State. Comparison of Genetic Profiles of Adipose Tissue in Identical Twins . . in wounds undergoing negative pressure wound therapy. Nate . Stearrett. (presentation format shamelessly stolen from Jeff . Elhai. ). Chronic Wounds. http://wjes.biomedcentral.com/articles/10.1186/1749-7922-2-10. . Differential . expression, clustering, networks, and functional enrichment. STEMREM 201 Fall 2012. Aaron . Newman, Ph.D.. 10/17/12. A . genomics approach . to . biology involves…. A plethora of . Draw 8 boxes on your paper. Gene regulation accounts for some of the phenotypic differences between organisms with similar genes.. 2005-2006. Gene regulation in bacteria. Control of gene expression enables individual bacteria to adjust their metabolism to environmental change. Keyboard. (Encoding). Disk. (Storage). Monitor. (Retrieval. Sequential Process. Studying memory. Memory. Persistence of learning over time through the encoding, storage, and retrieval of information. 5 Research-ByEdward KangToo often people imagine that long hours of studying are the best path to being a model straight-A student Yetresearch showsthat highly successful students actually spend less analysis - outliers. V2: batch effects. V3: data imputation. What . is measured by microarrays?. Microarray normalization. Differential gene expression (DE) analysis based on microarray data. Detection of outliers. We performed a genome-wide analysis of gene expressionto identify germline- and sex-regulated genes.Using mutants that cause defects in germ cell proliferationor gametogenesis, we identiÞed sets of g Pearley Chinta and Juliet V. Spencer . Abstract. Methods. Results. Conclusions. HCMV is a widespread pathogen in the general population and can cause severe disease in immune-compromised hosts. HCMV manipulates immune responses in several ways, one of which includes encoding genes with homology to host chemokine receptors. HCMV US27 encodes a chemokine-like receptor that stimulates host gene expression. While, no chemokine ligand has been identified for US27, it is constitutively active. US27 stimulates the gene expression of antioxidant response element (ARE) regulated genes by activation of the transcription factor nuclear respiratory factor 1 (NRF-1). The goal of this project is to identify specific host and viral genes that are regulated by US27. Increased expression of antioxidant genes is likely to benefit virus infection and enable more progeny virus to be produced. Thus, a better understanding of the US27 function has the potential to lead to the development of novel antiviral therapies necessary to treat HCMV infection. . What is measured by microarrays?. Microarray normalization. Differential gene expression (DE) analysis based on microarray data. Detection of outliers. RNAseq. data. DE analysis based on . RNAseq. data. Venet. , et al.. PLoS. Computational Biology, 2011. Molly Carroll. Biomedical Research Methods. Characterize mechanism in the model. Derive a marker that changes when the mechanism is altered. Show .