PPT-Integrative plasmid vectors for zinc-inducible gene express

Keith Herbert Synthetic Biology with Cyanobacteria Cox PA Banack SA Murch SJ et al Diverse taxa of cyanobacteria produce betaN methylamino Lalanine a neurotoxic amino acid Proc. PLASMID VECTORS. . . Cloning into a Plasmid. Bacteria are useful hosts.. They are easily grown. They are cheap to grow. They grow fast. They are easily manipulated in the laboratory. DNA can be inserted - transformation. Dr. E. What is a plasmid?. Small double stranded circular DNA. Naturally found in bacteria. It is completely separate form bacterial chromosomal DNA. It independently self replicates . May give bacteria a . INTRODU. C. TION. Gene transfer is to . transfer . a gene from one DNA . molecule . to another . DNA molecule.. The . directed desirable . gene . transfer . from one organism to another . and . the subsequent . A vehicle used to transfer genetic material such as DNA sequence from the donor organism to target cell of recipient organism(Host cell).. Also referred as carrier of DNA sequence.. Also referred as cloning vehicle or cloning DNA.. Jasmin . Sutkovic. Principles . of cloning, vectors and cloning strategies. by . Jasmin SUTJOVIC. Principles of cloning, vectors and cloning strategies. DNA CLONING. DNA cloning is a technique for reproducing DNA fragments. . . Hameed. Abdullah. (. or . rDNA. ) is made by combining DNA from two or more sources. In practice, the process often involves combining the DNA of different organisms. . The . process depends on the ability of cut, and re-join, DNA molecules at points identified by specific sequences of nucleotide bases called restriction sites. DNA fragments are cut out of their normal position in the chromosome using . Concept and basic steps in recombinant DNA technology and gene cloning. -1-. Cloning Vectors:. DNA molecule which is able to . replicate several time. in its self when a foreign DNA is integrated . and . Technology AQC - 321 Dr. Mamta Singh Assistant Professor COF (BASU), Kishanganj Recombinant DNA Technology... Definition : It is a technology of joining together of DNA molecules from two different s DNA Cloning . DNA cloning. . is process of making several identical copy of a gene or gene fragment. . Cloning. is a . natural process. . in biology where genetically identical individuals are produced by asexually reproducing organisms such as bacteria, insects or plants. In . and phages . and are known as . phasmids. or, if they . contain . an . M13 . ori region. , phagemids. .. 1. . Phagemid. . vectors are plasmids having a small segment of a filamentous phage M-13, . fd. A vector is a DNA molecule that has the abil­ity to replicate autonomously in an appropri­ate host cell and into which the gene of inter­est (a foreign genetic sequence) is integrated. When we insert a foreign genetic sequence into the vector the aim is either to obtain numer­ous copies of the gene of interest or to obtain the product of that.. Rubanraj. . Dept of Mathematics. St. Joseph’s College. Trichy. Vectors . Vector is an agent that can carry a DNA fragment into a host cell in which it is capable of replication. If it is used only for reproducing the DNA fragment, it is called a . by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. . T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. Unit 2. Vector:. Single celled, and easy to grow like bacteria. Yeast strains are genetically well characterized. Detailed genetic maps are. available for . Saccharomyces. . cerevisiae. and . Schizosaccharomyces. .