PPT-Integrative plasmid vectors for zinc-inducible gene express

Keith Herbert Synthetic Biology with Cyanobacteria Cox PA Banack SA Murch SJ et al Diverse taxa of cyanobacteria produce betaN methylamino Lalanine a neurotoxic amino acid Proc. Ben Kligler, M.D., Patricia Lebensohn, M.D., Sally Dodds, Ph.D., Raymond Teets, M.D., & Victoria Maizes, M.D.. Presentation Objectives. The objectives of this presentation are to:. Describe the function of formative evaluation for curriculum development.. PLASMID VECTORS. . . Cloning into a Plasmid. Bacteria are useful hosts.. They are easily grown. They are cheap to grow. They grow fast. They are easily manipulated in the laboratory. DNA can be inserted - transformation. TAP- NFCPA. Alyssa Wostrel, MBA . Executive Director, IHPC. October 9, 2015. Disclaimer . Neither my spouse nor I have any conflict of interest or financial commitments regarding this presentation and its content. . Dr. E. What is a plasmid?. Small double stranded circular DNA. Naturally found in bacteria. It is completely separate form bacterial chromosomal DNA. It independently self replicates . May give bacteria a . Ben Kligler, M.D., Patricia Lebensohn, M.D., Sally Dodds, Ph.D., Raymond Teets, M.D., & Victoria Maizes, M.D.. Presentation Objectives. The objectives of this presentation are to:. Describe the function of formative evaluation for curriculum development.. Any vector can be resized by multiplying it by a real number (scalar).. Multiplying by positive scalar changes magnitude only.. Multiplying by a negative scalar changes the magnitude and its direction.. The value of an integrative leader Professional DEVELOPMENT Support request: LEADERSHIP PROGRAM IN INTEGRATIVE HEALTHCARE AT DUKE UNIVERSITY Your organization’s logo here Submitted by: YOUR NAME DATE INTRODU. C. TION. Gene transfer is to . transfer . a gene from one DNA . molecule . to another . DNA molecule.. The . directed desirable . gene . transfer . from one organism to another . and . the subsequent . retardation. SIK2. Smooth . muscle . relaxation. Melanogenesis. SIK2 inhibitor. Anti hair graying. WT. KO. Resistance to. Stroke. Dementia . Skin. whitening. SIK3. glucose/. fatty acid. cholesterol/. A vehicle used to transfer genetic material such as DNA sequence from the donor organism to target cell of recipient organism(Host cell).. Also referred as carrier of DNA sequence.. Also referred as cloning vehicle or cloning DNA.. Concept and basic steps in recombinant DNA technology and gene cloning. -1-. Cloning Vectors:. DNA molecule which is able to . replicate several time. in its self when a foreign DNA is integrated . and . DNA Cloning . DNA cloning. . is process of making several identical copy of a gene or gene fragment. . Cloning. is a . natural process. . in biology where genetically identical individuals are produced by asexually reproducing organisms such as bacteria, insects or plants. In . and phages . and are known as . phasmids. or, if they . contain . an . M13 . ori region. , phagemids. .. 1. . Phagemid. . vectors are plasmids having a small segment of a filamentous phage M-13, . fd. learning objectives. Understand what is meant by genetic engineering.. Understand the steps involved.. Understand what restriction enzymes do.. Understand why sticky ends are important.. success criteria.