PPT-Blood stain analysis

Objective to understand how blood stains can give forensic scientists information about a crime blood Blood is what moves oxygen around our body from the lungs to the organs that need oxygen and then back the lungs for more oxygen. Staining total protein before specific protein de tection techniques provides an assessment of protein transfer efficiency and makes it possible to detect contaminating proteins in the sample and to compare the sample with molecular weight standards Figure 1. CD3+CD4+ live61%40%CD3+CD4+ no discriminationStat5(pY694) Alexa Fluor Gram Stain Reaction. The Gram stain reaction, named after the Danish bacteriologist Hans Christian Gram, was originally devised in 1882.. Gram staining is based on the ability of the bacterial cell wall to retain the crystal violet dye. . A stain is a substance that adheres to a cell, giving the cell color. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . Griffin. Bloodstain patterns. The patterns left by falling, projected, or smeared blood can help the forensic investigator interpret and reconstruct what has happened at a crime scene.. Observation of the position and shape of stain patterns can give information such as:. Practical Hematology Lab. Making Blood Smear. - LAB 1 -. I- Preparation Of Blood Smear. There are three types of blood smears: . The cover glass smear.. The wedge smear .. The spun smear.. The are two additional types of blood smear used for specific purposes. Directions: Identify each type of blood stain/spatter pictured. 1. What type of bloodstain?. 2. What type of blood stain?. 3. What type of blood stain?. 4. . . What type of blood stain?. 5. What type of blood stain?. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . organisms. They are used to differentiate different types of organisms or to view specific parts of organisms. 1. What type of bloodstain?. 2. What type of blood stain?. 3. What type of blood stain?. 4. . . What type of blood stain?. 5. What type of blood stain?. 6. What type of blood stain?. 7. type of blood stain?. tissues, cells, cell components, cell contents, or microorganisms.. *The stain may be . natural or synthetic. . . *The object stained is called the . substrate. . . *Staining facilitates the observation of a substrate by introducing . Blood vessels that carry blood from the heart The tiniest blood vessels Parts of the respiratory system refractile. body within the vegetative cells. Procedure: (fig.10) . 1- Prepare a fixed smear.. 2- Flood the smear with 5% malachite green and let the stain react for 1 minute .Using your . bunsen. burner, heat the top of the stained slide until steam rises but do not let it boil. This step is performed by passing the burner back and over the slide for about 5 minutes.. 10. Learning Objectives—Level l . At the end of this unit of study, the student should be able to:. List the assays typically included in the complete blood count (CBC).. Describe how to properly identify a patient prior to blood collection.. Fixatives used in histopathology. 2. Staining. Differential staining. Gram’s staining. Vital staining. Trypan. blue- dead cells take up the stain . i.e. stain positively and live cells . Exclude the stain or stain negatively.