PPT-Chapter 6 Restriction enzyme cloning

Plasmid 1 Gene Plasmid 1 Gene Restriction Endonuclease Plasmid 2 Gene Restriction Endonuclease Ligation Gene A plasmid used for bluewhite selection 2 Full size 2961 bp Each cell contains only . And 57375en 57375ere Were None meets the standard for Range of Reading and Level of Text Complexity for grade 8 Its structure pacing and universal appeal make it an appropriate reading choice for reluctant readers 57375e book also o57373ers students Unit 2. Module 2 – Biotechnology and Gene Technologies. Learning Objectives. Understand how animals can be cloned.. Understand what embryo splitting is.. Understand what nuclear transfer is.. Understand what non-reproductive cloning is.. An Introduction to Restriction Enzymes & Gel Electrophoresis. Objectives. Understand the use of restriction enzymes as biotechnology tools. Become familiar with principles and techniques of . agarose. Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . describe the production of natural clones in plants using the example of vegetative propagation in elm trees; . describe the production of artificial clones of plants from tissue culture;. discuss the advantages and disadvantages of plant cloning in agriculture (HSW6a, 6b, 7c); . Dr. Kevin Ahern. Protease Mechanisms. Aspartic Acid. Aspartic Acid. Histidine. Cysteine. Electron rich. Metal. Other Protease Families. Cysteine. Aspartyl. Metallo-. Papain. Cathepsin D. Carboxypeptidase A. Recombinant . DNA technology. Methods . used to join together (recombine) different DNA segments that are not found together in nature.. This technique is used in genetic analysis to serve several applications:. Gene cloning and other techniques, collectively termed DNA technology, can be used to manipulate and analyze DNA and to produce useful new products and organisms.. In genetic engineering, bacterial restriction enzymes are used to cut DNA molecules within short, specific nucleotide sequences (restriction sites), yielding a set of double-stranded DNA fragments with single stranded sticky ends.. A vehicle used to transfer genetic material such as DNA sequence from the donor organism to target cell of recipient organism(Host cell).. Also referred as carrier of DNA sequence.. Also referred as cloning vehicle or cloning DNA.. Objectives. -Understand how Restriction Enzymes digest DNA.. -Know how to construct a . pAMP. . (plasmid) or gel.. -Given the size of fragments, gel, know how to construct a restriction map.. -Given a restriction map know how to construct a gel.. What are restriction endonucleases (REs)?. How can REs be used to identify DNA molecules?. How can I find RE recognition sites in the . MET. plasmids? . Restriction endonucleases are part of a bacterium’s defense against invaders. A r t i c l e b y - , W e s t B e n g a l , I n d i a Emailed by Monazza Shahab A r t i c l e b y - M o n a z z a S h a h a b , W e s t B e n g a l , I n d i a CONSTRUCTION OF PARTIAL GENOMIC LIBRARY HOD Botany. MD College. Parel. ,. Mumbai-12 . Gene Cloning. Gene cloning.  is the process in which a . gene.  of interest is located and copied (. cloned. ) out of DNA extracted from an organism. .