PPT-L4: Effect of pH on Amylase Rate of Reaction

Learning Objectives Required Practical 4 Investigate the effect of pH on the rate of an enzyme controlled reaction Required Practical 4 We are going to investigate the effect of pH on the enzyme action of amylase. Structures and Properties. Halogen is connected to a tetrahedral carbon. The carbon-halogen bond is polarized with the carbon having a partial positive charge and the halogen having a partial negative charge. Chapter 1. Reaction Rates. What makes “superglue” bond instantly while Elmer’s glue does not?. What factors determine how quickly food spoils?. Why do “glow sticks” last longer when stored in the freezer?. Learning Objectives. Learn how the rate of an enzyme controlled reaction is measured.. Learn how temperature affects the rate of an enzyme controlled reaction.. Learn how pH affects the rate of an enzyme controlled reaction.. . Temperature effects the rate of reaction, cell metabolism, nutritional requirement by the cell and the biomass concentration. . 2 fold increase in the rate of reaction with every 10*C rise in temperature.. Objectives. To monitor the progress of an enzyme catalyzed reaction (Acid phosphatase).. To determine the initial rate of the reaction (. V. i. ).. Important terms and points:. Enzyme kinetics. The central approach for studying the mechanism of an enzyme-catalyzed reaction is to study enzyme kinetics. . Halogen is connected to a tetrahedral carbon. The carbon-halogen bond is polarized with the carbon having a partial positive charge and the halogen having a partial negative charge. The size of the halogen increases as you move down the group. Reaction Summary : S N 2, E2, S N 1/E1 Rate Competition: S N 2, S N 1/E1, E2 S N 2 S N 1 E1 E2 Optimize E2 rate: Factor 1: 3 o > 2 o >>1 o Factor 2: Strong Base: Factor 3: Good LG-weak CB Amylase . AMYLASE is an enzyme that is found in our bodies that functions to help the body in the digestion food. Amylase is found in saliva and in the pancreas.  . Amylase catalyzes the hydrolysis (breaking down) of starch, glycogen and related polysaccharides into more simple and readily usable forms of sugar. . In this experiment, we will continue to study acid phosphatase kinetics.. Objective:. To establish the relationship between enzyme concentration and the rate of an enzyme catalyzed reaction.. The reaction rate will increase as the concentration of enzymes is increased but there must be a . 322 BCH. . . Exp. (6). In this experiment, we will continue to study . acid phosphatase . kinetics.. Objectives. To establish the relationship between pH and the rate of an enzyme catalyzed reaction.. CLS 282. Haifa Altwijri –Daheeya Alenazi. 1442 H. introduction. In our bodies, there are organic and inorganic compounds.. Organic compounds include protein, fat, carbohydrates, and nucleic acids. Which are the major classes in human body…. Bio-Rad Biotechnology . Explorer™ Biofuel Enzyme Kit. Instructors - Bio-Rad Curriculum and Training Specialists. Sherri Andrews, Ph.D. ., Eastern US. sherri_andrews@bio-rad.com. Damon . Tighe. , . amylase. INTRODUCTION. *Amylase found in pancreas and salivary gland.. It is break down the polysaccharide into monosaccharide as this equation( General principle):. Starch amylase maltose + Glucose. Stoichiometry. (identity and relative amounts of reactants and products).. Spontaneity (feasibility of the reaction, based on thermodynamics).. Speed…KINETICS (reaction rates).. Stopping…when will the reaction stop? (Equilibrium-next chapter).