Learning Objectives Required Practical 4 Investigate the effect of pH on the rate of an enzyme controlled reaction Required Practical 4 We are going to investigate the effect of pH on the enzyme action of amylase ID: 908717
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Slide1
L4: Effect of pH on Amylase Rate of Reaction
Learning Objectives:
Required Practical 4
: Investigate the effect of pH on the rate of an enzyme controlled reaction.
Slide2Required Practical 4
We are going to investigate the effect of pH on the enzyme action of amylase.
What does amylase digest?
How do we test for starch?
How will we test whether pH has an effect on enzyme function?
How will you compare the rates of the reaction?
Slide3Required Practical 4
Amylase
breaks down
starch
into
glucose
.
We can
test for starch
using
iodine solution
(turns blue/black if starch is present).
We will
time
how long it takes for the starch to be
completely digested
by the amylase under
different pH
conditions. The longer the time, the slower the rate.
Slide4Effect of pH on Amylase Rate of Reaction
Purpose: We are going to investigate how pH effects the rate of amylase catalysed break down of starch.
Hypothesis:
Independent variable (we change) –
Dependent variable (we measure) –
Control variables (fair test) –
Slide5Effect of pH on Amylase Rate of Reaction
Purpose: We are going to investigate how pH effects the rate of amylase catalysed break down of starch.
Hypothesis: make a prediction
Independent variable (we change) – pH
Dependent variable (we measure) – the rate (measure time taken to fully break down starch)
Control variables (fair test) – amount of starch, amount of amylase, temperature
Slide6Homework:
Plot a graph of your results from the practical.
Write a one paragraph conclusion.