PPT-L4: Effect of pH on Amylase Rate of Reaction

Learning Objectives Required Practical 4 Investigate the effect of pH on the rate of an enzyme controlled reaction Required Practical 4 We are going to investigate the effect of pH on the enzyme action of amylase. . Energy and Chemical. Reactions. The reaction rate for a chemical system is defined as the time that it takes for the reactants to be converted into products. The faster this occurs, the faster the chemical reaction, and vice versa. . (CRE) is the field that studies the rates and mechanisms of chemical reactions and the design of the reactors in which they take place.. Lecture. 14. Today’s. . lecture. Pseudo Steady State Hypothesis (PSSH). (CRE) is the field that studies the rates and mechanisms of chemical reactions and the design of the reactors in which they take place.. Lecture. 14. Lecture 14 – Thursday 2/24/2011. Pseudo Steady State Hypothesis (PSSH). Chapter 1. Reaction Rates. What makes “superglue” bond instantly while Elmer’s glue does not?. What factors determine how quickly food spoils?. Why do “glow sticks” last longer when stored in the freezer?. . Temperature effects the rate of reaction, cell metabolism, nutritional requirement by the cell and the biomass concentration. . 2 fold increase in the rate of reaction with every 10*C rise in temperature.. Lecture 14 . Reaction Rates. Recall that we defined the rate of reaction as the rate of production of the products, or equivalently, the rate of consumption of the reactants.. Reaction rates for elementary reactions depend on:. Food biochemistry and food processing. 2012. Simpson B.K. (Ed.) 2. nd. . edition,. John Wiley & Sons, . Inc.. Enzymes in food processing. Advantages.. Enzymes are proteins with powerful catalytic . Reaction Summary : S N 2, E2, S N 1/E1 Rate Competition: S N 2, S N 1/E1, E2 S N 2 S N 1 E1 E2 Optimize E2 rate: Factor 1: 3 o > 2 o >>1 o Factor 2: Strong Base: Factor 3: Good LG-weak CB 322 BCH. . . Exp. (6). In this experiment, we will continue to study . acid phosphatase . kinetics.. Objectives. To establish the relationship between pH and the rate of an enzyme catalyzed reaction.. GROUP # 5 . SUBMITTED TO : . Dr. . Hajra. . Sadia. . GROUP MEMBERS :-. Maarej. Khan. Zara . Taimoor. . Rameeza. Hassan. Maryam. . Jadoon. Hanzala. Jahangir. Maham. . Nadeem. Shehrbano. . Akhtar. .. Answer True or False to the following statements about enzymes: . True or False?. Enzymes are Biological Catalysts. True or False?. Enzymes are made mainly of lipids. True or False?. The names of Enzymes usually end in ‘’. Kim . Gernert. Emory University, Atlanta, GA. Vedham. . Karpakakunjaram. Montgomery College, Rockville, MD. Target Audience. Students enrolled in Principles of Biology I (BI 107) and II (BI 108). Human salivary amylase: used in one of our lab modules, so students are familiar with this enzyme and its function. a. A. . + . b. B. → . c. C. + . d. D. Rate = . k . [. A]. m . [. B]. n . The . term . k. . is the . rate constant. , which is specific for a given reaction at a given temperature. .. Hands-on . session. : . alpha. . amylase. alpha. . amylase. . as. a . saliva. . based. . biomarker. . for. . the. . analysis. . of. stress in a motivational fit-situation. Most . previous.