P hosphate A phosphate an inorganic chemical is a salt of phosphoric acid It consists of one central phosphorus atom surrounded by four oxygen atoms in a tetrahedral arrangement The phosphate ion carries a negative three formal charge ID: 1011623
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1. The Colorimetric Estimation of Inorganic Phosphate
2. A phosphate, an inorganic chemical, is a salt of phosphoric acidIt consists of one central phosphorus atom surrounded by four oxygen atoms in a tetrahedral arrangement. The phosphate ion carries a negative three formal charge Introduction
3. phosphorus is found as a free phosphate ion in solution and is called inorganic phosphate, to distinguish it from phosphates bound in various phosphate esters. Inorganic phosphate is generally denoted Pi
4. Phosphate are most commonly found in the form of adenosine phosphates, (AMP, ADP and ATP) and in DNA and RNA
5. PrincipleThe measurement of inorganic phosphate must be one of the comments determinations carried out in a biochemistry lab. It is certainly surprising how frequently such a measurement is required, and the production of a standard curve is a useful exercise for checking the validity of Beer’s Law.
6. Inorganic phosphate reacts with ammonium molybdate in an acid solution to form phosphomolybdic acid. A reducing agent such as metol is added which reduces the molybdenum to give a blue colour but does not affect the uncombined molybdic acid.
7. Pi + ammonium molybdate phosphomolybdic acid phosphomolybdic acid + metol(reducing agent) blue colourMetol not combine with excess of molybdic acid Acid solu
8. Method
9. Blank Std1 Std2 Std3 Std4 Std5 unTCA1ml0.9ml0.7ml0.5ml0.3ml __St.sol(w.s)_ 0.1ml0.3ml0.5ml0.7ml1ml_Copper acetate buffer(pH4)3ml3ml3ml3ml3ml3ml3mlAmmonium molybdate0.5ml0.5ml0.5ml0.5ml0.5ml0.5ml0.5mlMetol0.5ml0.5ml0.5ml0.5ml0.5ml0.5ml0.5mlUnknown_____ _1mlMix, incubate at RT for 10 min. And then read at 880nm.
10. CalculationC1 X V1 = C2 X V2
11. IntroductionInorganic phosphate react with ammonium molybdate in an acid solution to form phosphomolybdic acid.A reducing agent such as metol is added which reduces the molybdenum to give a blue colour but does not affect the uncombined.