PPT-Nucleosome positioning and

3D genome organisation Vladimir Teif BS222 Genome Science Lecture 6 Module structure Genomes sequencing projects and genomic databases VT Sequencing technologies VT What is a gene . & . RE-POSITIONING STRATEGIES . ADOPTED BY RIM. . FOR. . 1. A BRIEF INTRO OF BlackBerry…. BlackBerry is a line of wireless handheld device developed by the Canadian company, Research In Motion (RIM).. : what, why and where?. Rob Brewster. Outline. What is a . nucleosome. ?. - how is DNA packaged/organized in Eukaryotes?. Why do . nucleosomes. form?. . - DNA is stiff, how do ~100 . bp. loops form so readily?. May 2010. Nucleosome. Positioning. & . Transcription Factor . Identification. Outline. Basic Concept. Nucleosome. Positioning and Gene Regulation. General Transcription Mechanism. Genomic Organization of . Positioning can involve . brand. . name. , . image. , . packaging. , the way it is . delivered. but is usually a . combination. of these. - A stance. - An attitude. - A perception. - A point of view. Y. Lyse & Sonicate. IP. Reverse crosslinks. Total. Reverse crosslinks. Amplify. Amplify. Sequence. Sequence. Immuno. -precipitation. Other controls for IP. (e.g., no antibody, non-specific antibody). What is Positioning?. What does a GPS tell you?. What is Positioning?. Positioning is where you are. In the mind of the consumer. Relative to you competition. Creates an image... a unique identity. Examples:. A genomic code for . nucleosome. positioning. Eran. Segal, Yvonne . Fondufe-Mittendorf. , . Lingyi. Chen, . AnnChristine. . Thåström. , . Yair. Field, Irene K. Moore, . Ji. -Ping Z. Wang, and Jonathan . Positioning. Developing a specific marketing mix to influence potential customers’ overall perception of a brand, product line, or organization in general.. What is Positioning?. The place a product, brand, or group of products occupies in consumers’ minds relative to competing offerings.. ADOPTED BY RIM. . FOR. . 1. A BRIEF INTRO OF BlackBerry…. BlackBerry is a line of wireless handheld device developed by the Canadian company, Research In Motion (RIM).. 1999 – Introduced a two-way pager. Chromatin-binding factorshistone macromolecules that bind either directly or indirectly to DNA.Transcription factorhistone protein that directly binds to DNA.Architectural proteinsProteins that have a Rob Brewster. Outline. What is a . nucleosome. ?. - how is DNA packaged/organized in Eukaryotes?. Why do . nucleosomes. form?. . - DNA is stiff, how do ~100 . bp. loops form so readily?. Where do . Positioning. & . Transcription Factor . Identification. Outline. Basic Concept. Nucleosome. Positioning and Gene Regulation. General Transcription Mechanism. Genomic Organization of . Nucleosomes. Don and Ada . Olins. in 1974. , and their existence and structure (as . histone . octamers. surrounded by approximately 200 base pairs of DNA. ) were proposed by . Roger Kornberg. .. . The role of the nucleosome as a . Chromatin organization marks . exon-intron. structure. [Schwartz S, Meshorer E, Ast G . Nat . Struct. Mol . Biol. , 2009 Sep;16(9):990-6]. . Preface:. Crystal structure of . nucleosome. core particle.