PPT-Unit 4: Regulating Gene Expression

Chapter 20 Genome Defense Figure 2001 Antisense RNA Can Base Pair With mRNA mRNA is normally made using the noncoding strand of DNA as a template Such mRNA is also known as sense RNA. Vibrio cholerae . HHMI 2011. Sara Fassio. Dr. Claudia H. ä. se. Dr. Yusuke Minato . Cholera. Infection of small intestine . Causes severe diarrhea and electrolyte loss. 3-5 million cases a year. 100,000- 120,000 deaths per year . What does the operon model attempt to explain?. the coordinated control of gene expression . in bacteria. bacterial resistance to antibiotics. how genes move between homologous regions of DNA. the mechanism of viral attachment to a host cell. Stephen Montgomery. smontgom@stanford.edu. montgomerylab.stanford.edu. @. sbmontgom. Identifying the genes and variants responsible for disease.. Monogenic to polygenic diseases. Rare to common variants. . Differential . expression, clustering, networks, and functional enrichment. STEMREM 201 Fall 2012. Aaron . Newman, Ph.D.. 10/17/12. A . genomics approach . to . biology involves…. A plethora of . Gene Expression. Gene Expression. Transcription. Splicing. Polyadenylation. mRNA Stability. Translation. Protein Stability. Controls on Protein Levels. Transcription Control - Prokaryotic Promoter. Transcription Control - Prokaryotic Promoter. Drosophila. Embryos using . lacZ. Transgenes. June 18. th. ABLE 2014. University of Oregon, Eugene. Cathy Silver Key. Julie Gates. Jessica Sawyer. Kirsten . Guss. Acknowledgements. Funding from . Roberta . analysis - outliers. V2: batch effects. V3: data imputation. What . is measured by microarrays?. Microarray normalization. Differential gene expression (DE) analysis based on microarray data. Detection of outliers. We performed a genome-wide analysis of gene expressionto identify germline- and sex-regulated genes.Using mutants that cause defects in germ cell proliferationor gametogenesis, we identiÞed sets of g  which is designed to allow . expression.  (. transcription.  and . translation. ) of the inserted section of . DNA. . The . vector.  carries a . promoter.  (normally inducible) on one side of the . Pearley Chinta and Juliet V. Spencer . Abstract. Methods. Results. Conclusions. HCMV is a widespread pathogen in the general population and can cause severe disease in immune-compromised hosts. HCMV manipulates immune responses in several ways, one of which includes encoding genes with homology to host chemokine receptors. HCMV US27 encodes a chemokine-like receptor that stimulates host gene expression. While, no chemokine ligand has been identified for US27, it is constitutively active. US27 stimulates the gene expression of antioxidant response element (ARE) regulated genes by activation of the transcription factor nuclear respiratory factor 1 (NRF-1). The goal of this project is to identify specific host and viral genes that are regulated by US27. Increased expression of antioxidant genes is likely to benefit virus infection and enable more progeny virus to be produced. Thus, a better understanding of the US27 function has the potential to lead to the development of novel antiviral therapies necessary to treat HCMV infection. . What is measured by microarrays?. Microarray normalization. Differential gene expression (DE) analysis based on microarray data. Detection of outliers. RNAseq. data. DE analysis based on . RNAseq. data. Venet. , et al.. PLoS. Computational Biology, 2011. Molly Carroll. Biomedical Research Methods. Characterize mechanism in the model. Derive a marker that changes when the mechanism is altered. Show . Developing Ovule,. . Embryo and Endosperm. The 2. nd. . International . Conference on Transcriptomics. Jianbo. Wang. Wuhan University, China. Background. Materials and methods. Gene expression. . Leading TV Unit Manufacturer in Pune Innovative Designs, Superior Quality at Adeetya's Kitchen & Furniture https://adeetyas.com/tv-unit-manufacturers-in-pune.php