PPT-Synthetic approaches to transcription factor regulation and

Tim Johnstone BIOL1220 Spring 2010 Transcription Factor a protein that binds to specific DNA sequences to modulate the transcription of DNA to mRNA The transcription factor TATA binding protein blue bound to DNA red Image by David S . 7. 7 RNA Synthesis and Processing. Chapter Outline. Transcription in Prokaryotes. Eukaryotic RNA Polymerases and General Transcription Factors. Regulation of Transcription in Eukaryotes. RNA Processing and Turnover. (TZD) . in . adipocytes. Kyoung-Jae Won, . Ph.D.. Sep 8 . 2014 . . Genomics 2014. Non-coding RNA Annotation in ENSEMBL. tRNA. Mt-. tRNA. rRNA. scRNA. snRNA. snoRNA. miRNA. Misc_RNA. lincRNA. eRNA. (enhancer driven RNA). i. n Eukaryotes. Lecture #2. 1/23/17. References. . A few of the many insights from RNA polymerase structures. Cramer, P. (2002) . Multisubunit. RNA polymerases. . Curr. . Opin. . Struct. . Biol. Colloquium, March 9. RULES. Multiple choice. Only one correct answer. 30-60 seconds to answer. No textbook, computer, mobile phone, please. There will be a prize for the group with the most correct answers.. Gene Expression Group. 7/14/11. 2011 National Academies Northstar Institute for Undergraduate Education in Biology . Outline. Context. Review: Clicker Questions. Cell Differences (Think-Pair-Share). Regulation of Gene Expression (Mini-Lecture). Pre-transcriptional regulation. chromatin compaction . eg. . deacetylation. , . methylation. transcriptional initiation . ie. transcription factors to activate or repress. alternative promoters =. Outline. Objective. Mechanism. K. inase cascades and Phosphorylation/. dephosphorylation. . General. Learning/LTP. Synaptic strengthening. Control of gene expression. Transcription factors. Epigenetics. Structure of RNA. Major Classes of RNA. Transcription in Prokaryotes. Transcription in Eukaryotes. Post-transcriptional Processing of Eukaryotic mRNA. Transcriptional Regulation in Prokaryotes:. the Lac Operon as an example. synthesis). A. Prokaryotes: RNA polymerase, . Promoters – sigma factor. B. Eukaryotes: RNA polymerases, . Promoters – transcription factors. . 1 strand of . dsDNA. used as template to synthesize complimentary . © 2017 W. H. Freeman and Company. CHAPTER 28 . Regulation of Gene Expression. DNA elements that control transcription. Protein factors that control transcription. Lac. operon as a model for regulation. Copyright 2016 by the Rector and Visitors of the University of VirginiaSTAT3STAT3STAT3STAT3RNAABNucleus of a cellBackgroundProtein products made from genes will have specific functions in the cell One Translated By Emaan Chaudry. Adaptation to low oxygen conditions (hypoxia):. Transcriptional Responses. Regulation of the gene: . F45D3.4. How do we figure out what is causing the f45d3.4 gene to be expressed?. Positioning. & . Transcription Factor . Identification. Outline. Basic Concept. Nucleosome. Positioning and Gene Regulation. General Transcription Mechanism. Genomic Organization of . Nucleosomes. A eukaryotic transcription initiation complex consists of RNA polymerase II, . five. general transcription factors (GTFs), and a 20-subunit complex called Mediator (or . Srb. /Med). The CTD of RNA polymerase II anchors Mediator to the polymerase. Mediator allows RNA polymerase II to communicate with transcriptional .