PPT-In-depth Analysis of Protein Amino Acid Sequence and PTMs with High-resolution

Lian Yang 2 Baozhen Shan 1 Bin Ma 2 1 Bioinformatics Solutions Inc Canada 2 University of Waterloo Canada Problem Complete protein sequence coverage antibody confirmation. Formation of Aminoacyl tRNAs. Ribosome structure. Stages of Translation. Relationship between DNA, mRNA, and Protein Sequences. Translation and the Genetic Code. Amino acid structure. Four different groups are attached to the central carbon atom (. We live in a human-centric world.. Life exists outside our box.. Subtitle. Text. Shock & Holland (2007). For example, there is life deep down on the ocean floor.. C-DEBI . (Center for Deep Energy Biosphere Investigations). Alexey Onufriev, . Virginia Tech. www.cs.vt.edu/~onufriev. Proteins play key roles in a living system. Three (out of many many) examples of protein functions. Catalysis:. Almost all chemical reactions in a living cell are catalyzed by protein enzymes.. RNA. DNA . RNA. RNA. ( RIBONUCLEIC ACID ) . SINGLE STRANDED NUCLEIC ACID.. RNA CONTAINS RIBOSE (HYDROXYL GROUP ATTACHED TO THE PENTOSE RING) .. David Horn. Thermo Fisher Scientific . San Jose, CA. Outline. Challenges in the characterization of biopharmaceuticals. Introduction to the new generation of . Orbitrap. TM. . mass spectrometers. Confident intact antibody analysis using the Q . Polypeptides . are chains of amino acids linked together by condensation reactions. the main, or only, component in proteins. Some proteins are composed of only one polypeptide chain, while others are made of 2 or more. Objectives 82 Each of the 20 amino acids commonly found in proteins has an R (reactive) group with its own distinctive chemistry. R groups dier in their size, polarity, charge and bonding potent (N. utritionally. . Essential & Nutritionally Non Essential Amino . Acids ). Lecture : 4. Dr. . Shaimaa. . Munther. Nutritionally Essential & Nutritionally Non Essential Amino Acids . Introduction: . unbranched. polymer chains composed of numerous small subunits or monomers is called amino acids .. There are 20 amino acids from which 18 have same structure.. DEFINATION. STRUCTURE. Amino acids have a central chiral carbon atom called alpha carbon. Groups attached to carbon atom are: . INTRODUCTION. PROTEIN SEQUENCING. WHY PROTEIN SEQUENCING MATTERS?. HISTORY. STRATEGY. SEQUENCING METHODS. APPLICATIONS. INTRODUCTION. PROTEIN. Proteins are polymers of amino acid. Protein's structure & function depends upon amino acid sequence. The function of the different types of RNA in the cell is to facilitate the task of synthesizing proteins.. An activated . tRNA. with anticodon AGU bonds to serine at the acceptor stem.. Learning Goal . Daum LT, Canas LC, Smith CB, Klimov A, Huff W, Barnes W, et al. Genetic and Antigenic Analysis of the First A/New Caledonia/20/99-like H1N1 Influenza Isolates Reported in the Americas. Emerg Infect Dis. 2002;8(4):408-412. https://doi.org/10.3201/eid0804.010311. Explain the formation . of a protein.. Classify proteins according to their chemical structure.. Describe the classification of protein.. Name a food source for the following proteins: albumin . caseinogen. Proteins are naturally-occurring biopolymers comprised of amino acids. . The biological function of proteins is inherent in their three dimensional structure.. All the information required for correct folding of the protein into its functional .