Hind III Xho I His Tag ATG codon recombinant Abrin GAGCTC CGAATGAAGAC AAGCTT TGA codon pETAbrin 6139 bp Supplementary Figure 1 ID: 771223
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Hind III Xho I His -Tag ATG codon recombinant Abrin GAGCTC …… CGAAT.……………….…..…………………….GAAGAC ..... AAGCTT TGA codon pET-Abrin (6139 bp ) Supplementary Figure 1. Schematic representation of the designed plasmid containing the recombinant sequence for abrin protein. It shows the main characteristics of the plasmid construct such as the appropriate antibiotic for selection of positive clones, the multicloning site for the insertion of the recombinant Abrin (770 pb) between the Xho I – Hind III restriction enzymes, the respective start and stop codon sequences for the transcription of the insert, and the 6 histidine residues added for the subsequent purification of the recombinant protein. Supplementary Figure 1