Chemicals Solutions wwwenzymicalscom NewProt Kickoff meeting 25012012 Partner profile Enzymicals AG Dr Rainer Wardenga Startup history Spin off from the Institute of Biochemistry at the University of Greifswald ID: 225222
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Slide1
Enzymes .
Chemicals
. Solutions .
www.enzymicals.com
NewProt
Kick-off
meeting
: 25.01.2012
Partner
profile
Enzymicals
AG
Dr. Rainer WardengaSlide2
Start-up history
Spin off from the Institute of Biochemistry at the University of Greifswald
Founded in August 2009 as corporation
Management
Dr. Ulf Menyes
(CEO) Dr. Rainer Wardenga (CSO)
Supervisory Board
Prof.
Uwe Bornscheuer (Chairman) Dr. Dagmar Braun Dr. Jürgen EckOwnership structure Shareholders and strategic investor
1
Northern Germany,
Biotechnikum
Greifswald
Company Info
www.enzymicals.comSlide3
1
Company Info
www.enzymicals.com
Northern Germany,
Sinetific Campus Greifswald
Start-up history
Spin off from the Institute of Biochemistry at the University of Greifswald
Founded in August 2009 as corporation
Management
Dr. Ulf Menyes (CEO)
Dr. Rainer Wardenga (CSO)
Supervisory Board
Prof. Uwe Bornscheuer (Chairman)
Dr. Dagmar Braun Dr. Jürgen Eck
Ownership structure
Shareholders and strategic investorSlide4
Management and staff
Dr. U. Menyes (Chemist), CEO
Dr. R. Wardenga (Biologist and Biotechnologist), CSO
Dipl. S. Weber (Economist), finance and controlling
Biotechnologists (main focus molecular biology)
Biotechnologist (main focus biocatalysis)
Chemical technical assistant Biological technical assistant2
Company Info
www.enzymicals.com
Scientific staff
Company managementSlide5
Business Segments
3
Company Info
www.enzymicals.comSlide6
Institute of Biochemistry at the University of Greifswald (Prof. Bornscheuer)
B.R.A.I.N.
AG
HERBRAND
PharmaChemicals
GmbH
Institute of Chemistry at the University of Rostock (Prof. Kragl) Institute of Marine Biotechnology e.V. Greifswald
www.enzymicals.com
4
PartnersSlide7
Research & Development Along the Value Chain
www.enzymicals.com
5
Core competence in protein science and development of biocatalytic processSlide8
www.enzymicals.com
6
Research & Development Along the Value Chain
Identification of suitable catalysts
Method development and routine application of HPLC, CE, GC and GC-MS
Design and application of spectrophotometric assays
Synthesis of screening substrates
Screening of enzymes or strain collections Search in literature or pathway databases Search of known or novel motives in sequence databases
Amplification of novel genes from genomic DNA
De-novo genome sequencing and analysis Slide9
www.enzymicals.com
7
Cloning, expression and purification
Fully equipped molecular laboratories
State of the art expertise in molecular cloning
Huge repertoire of expression vectors
Well-founded knowledge of a range of recombinant expression systems Approved solutions for proteins which are difficult to express Screening of refolding conditions for aggregated proteins Production capacity up to 30
L-scale
Catalysts are available in different grades of purity
Purification via affinity-chromatography or fusion-tags
Research & Development Along the Value ChainSlide10
www.enzymicals.com
8
Cloning, expression and purification in collaboration with B.R.A.I.N AG
Peptide and Protein Production in
Escherichia coli / Bacillus sp. Pseudomonas stutzeri / Pichia pastoris
Streptomyces sp.
Aspergillus sp.
Production Systems:Optimized Production of Peptides or Proteins in Industrial Scale
Research & Development Along the Value ChainSlide11
www.enzymicals.com
9
Biochemical, physiochemical and structural characterization
Determination of catalytic properties and kinetic parameters
Characteristics in temperature- and pH-profiles
Investigation of storage and process stability
Properties via gel-electrophoresis and blotting Secondary structure via CD-spectrophotometry Screening for crystallization conditions via commercial kits
Access to MALDI-TOF, 2D-gel-electrophoresis
General immobilization studies
Research & Development Along the Value ChainSlide12
www.enzymicals.com
10
Protein engineering
Selection of best engineering approaches for designated targets
Approaches often inspired by structural alignments or molecular modeling
Homology-model construction
and hot-spot
identification Rational design of variants by site-directed mutagenesis Directed evolution with high quality variant libraries Selection assays and library screening with high-throughput systems HT-screening of mutant-libraries
Access to 3-laser flow cytometer with cell sorter
Research & Development Along the Value ChainSlide13
www.enzymicals.com
11
Application of best catalysts paired with optimized chemistry
Innovative synthetic routes using organic synthesis and biotransformation
Custom manufacturing of fine and special chemicals up to the multi-kg scale
Catalysis in multi-phase systems or ionic liquids
Membrane processes for catalyst recovery and downstream processing
Research & Development Along the Value ChainSlide14
www.enzymicals.com
12
Application of best catalysts paired with optimized chemistry
Innovative synthetic routes using organic synthesis and biotransformation
Custom manufacturing of fine and special chemicals up to the multi-kg scale
Catalysis in multi-phase systems or ionic liquids
Membrane processes for catalyst recovery and downstream processing
Scope of our biocatalytic tollbox
Research & Development Along the Value ChainSlide15
www.enzymicals.com
13
NewProt
:
Objectives
(R)-selective amine-transaminases
Catalyze the transfer an amino group from a primary amine to a
prochiral ketone or aldehyde Usable for the resolution of racemic amines or the synthesis of chiral amines Pyridoxal-5‘-phosphate dependent
Typical amines : alanine, ethylamine, 1- and 2-propylamine, 1- and 2-butylamine ….Slide16
www.enzymicals.com
14
(R)-selective amine-transaminases
Catalytic tool box for the evaluation of synthesis routes
Conversion of aliphatic,
arylaliphatic
and aromatic ketones with strict (
R
)-selectivity pH-optima range between 7.0 and 8.5 Alanine serves as amine donorProducts obtained by asymmetric synthesis with ECS-ATA01-07
.
NewProt
:
ObjectivesSlide17
www.enzymicals.com
15
Baeyer-
Villiger
monooxygenases
Flavoenzymes
from the class of oxidoreductases Oxidation of linear, cyclic and aromatic ketones to esters or lactones Usable with cofactor regeneration or as whole cell system Known applications:
Biooxidation
of functionalized cycloketones
Biooxidation of bicyclic ketones or chiral
b-substituted cycloketones Kinetic resolution of racemic a-substituted cycloketones
Regiodivergent
biooxidation of fused bicyclobutanones
NewProt
:
ObjectivesSlide18
www.enzymicals.com
16
Baeyer-
Villiger
monooxygenases
Catalytic tool box for the evaluation of synthesis routes
Baeyer-
Villiger oxidation of aromatic and aliphatic ketones, able to perform sulfur oxidations High regio- and enantioselectivity, NAD(P)H-dependent
Scope of the ECS
biooxidation
-toolbox. Examples of products obtained from the corresponding
ketones with ECS-M01-07.
NewProt
:
ObjectivesSlide19
www.enzymicals.com
Thank you for your attention
Enzymicals
AG
,
Walther-Rathenau-Straße 49a
17489 Greifswald
Fon: +49(0)3834 515-47-0
Fax: +49(0)3834 515-47-3