PPT-Lab (3): Column chromatography of

Carotenoids TA Nouf Alshareef KAUFaculty of Science Biochemistry department Analytical biochemistry lab Bioc 343 2012 nfshareefhotmilcom Background Carotenoids are organic fatsoluble pigments that . Invention of Chromatography. Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . Principle and Definition. Principle. The principle of chromatographic separation is very simple. The process is achieved by . distributing the substances. to be separated between a . mobile phase. and a . Hazards and Controls. Flash Chromatography. Hazard . Control. Spilled solvent could contact skin. Solvent could penetrate through gloves and contact skin . Wear goggles, gloves (nitrile), and lab coat. Instrumentation. Invented by a Russian Botanist Mikhail . Tswett. in 1903 . He used chromatography . . to separate the colour . . pigments in plants from . . one another. Chromatography is a separation technique in which a mobile phase carrying a mixture moves in contact with a selectively adsorbent stationary phase. Stationary phase. Mobile phase. Separation AND Characterization. Chromatography Basics. Based on different affinities for stationary and mobile phases. Silica gel: polar, water-covered surface. Compound(s). Introduction. Gas chromatography is used in many research labs, industrial labs (quality control), forensic (arson and drug analysis, toxicology, etc.), environmental labs (water, soil, air), and even in the popular TV culture (crime shows like NCIS (. BASICS OF CHROMATOGRAPHY Prepared by DR: Sherif Abdel- Naby Abdel- Gawad PhD Analytical Chemistry-Faculty of Pharmacy - Cairo University History of Chromatography Mikhail Tswett is credited with Announcements I. Homework Set 3 – due today. Quiz 5 (Ave on Q5 was 1.75. ) . –. Final . Exam. Thursday, May 18. th. 12:45-2:45. About 50% Review/50% New Material. Allowed 1 8.5” x 11” sheet of notes (no equations provided). , All right reserved 123 International Journal of Agriculture Innovations and Research Volume 6 , Issue 1 , ISSN (Online) 2319 - 1473 Gas Chromatography : Principles , Advantages and Applications i Course: PG P a athshala - Paper 3: Techniques in Molecular Biophysics Module 1 5 : Gas chromatography : principles and application TABLE OF CONTENTS 1. Learning Outcomes 2. Introduction and overvi Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . Dr . Debalina. . Basu. Department of Microbiology. Surendranath. College. Paper Chromatography. Principle. What is Descending Paper Chromatography?. Descending paper chromatography is an analytical technique in which the mobile phase moves downward through the stationary phase. In other words, in this method, the development of the paper occurs due to the movement of the solvent downwards on the paper. Therefore, the solvent reservoir should be at the top of the paper. In this process, the movement of the solvent is governed by gravity as well as capillary action.. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. Separation Technique, . Chromatography.  . For Postgraduate (. Master's degrees).  . Lecture No. 1.  By . Asst. Prof. Dr. Layla Salih Al-Omran . Chromatography.  . Background . Chromatography is a technique in which substance are separated, purified and identified from a mixture for qualitative and quantitative analysis. .