PPT-Lab (3): Column chromatography of

Carotenoids TA Nouf Alshareef KAUFaculty of Science Biochemistry department Analytical biochemistry lab Bioc 343 2012 nfshareefhotmilcom Background Carotenoids are organic fatsoluble pigments that . Supervisor:. Anton Zavialov. By: . Elham Barazeghi. Mehrafarin Ramezani. Affinity Chromatography:. Separation of proteins by reversible interaction of proteins and specific ligand in matrix of column. Chapter 4. Best Broken into four categories. Theoretical Background. Gas Chromatography. HPLC. Quantitation, Calibration, Standardisation and Validation. Theory. Review of Partitioning. You need to be aware of the following concepts in order to have any idea about this chapter!. Hazards and Controls. Flash Chromatography. Hazard . Control. Spilled solvent could contact skin. Solvent could penetrate through gloves and contact skin . Wear goggles, gloves (nitrile), and lab coat. Instrumentation. Invented by a Russian Botanist Mikhail . Tswett. in 1903 . He used chromatography . . to separate the colour . . pigments in plants from . . one another. Chromatography is a separation technique in which a mobile phase carrying a mixture moves in contact with a selectively adsorbent stationary phase. Stationary phase. Mobile phase. Separation AND Characterization. Chromatography Basics. Based on different affinities for stationary and mobile phases. Silica gel: polar, water-covered surface. Compound(s). Introduction. Gas chromatography is used in many research labs, industrial labs (quality control), forensic (arson and drug analysis, toxicology, etc.), environmental labs (water, soil, air), and even in the popular TV culture (crime shows like NCIS (. Instrumental Analysis Instrumentation Paper Chromatography GC – Gas Chromatography HPLC- High Performance Liquid Chromatography IR – Infrared Spectroscopy UV-Vis Spectroscopy MS – Mass Spectrometry chromatography. . (HPLC). Dr. J. Rajesh. Asso. . Prof. Dept of Chemistry. MSEC, Kilakarai. A. f. orm . o. f. s. e. pa. r. a. t. e. ,. c. o. l. u. m. n . identify,. ch. ro. m. a. to. gr. ap. h. y . . Chemistry. II. Gas. . Chromatography. Mobile . phase. : . inert. . gases. (. helium. , . nitrogen. , argon . etc. ). Stationary. . phases. : in . capillary. . column. Liquid (. siloxane. . polymers. A sample containing the materials to be separated is injected into the gas chromatograph. . Gas Chromatography. A mobile phase (carrier gas) moves through a column that contains a wall coated or granular solid coated stationary phase. . Announcements I. Homework Set 3 – due today. Quiz 5 (Ave on Q5 was 1.75. ) . –. Final . Exam. Thursday, May 18. th. 12:45-2:45. About 50% Review/50% New Material. Allowed 1 8.5” x 11” sheet of notes (no equations provided). Gas Chromatography Mass Spectrometry Dr Kersti Karu email: kersti.karu@ucl.ac.uk Office number: Room LG 11 Recommended Textbooks: - “ Analytical Chemistry ” , G. D. Christian, P. K. Dasgupta The word Chromatography is derived fro two Greek . words –. Chroma . means – colour and . graphein. . to write. . Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. . Chemistry. II. Chromatography. Real . samples. . are. . generally. . complex. . mixtures. . and. . substances. . interfere. . with. . each. . other. in . their. . analyses. . . Chromatography.