PPT-Microscopy and Magnification

1000 1000 1000 1000 mm Micrometre n m Nanometre Micrometre Millimetre 5 0005 0000005 1       1       1   3   7         05       0001 0000001 1000. Basic Introduction. Bob Ashley. 6-14-2013. Overview . Why electrons?. Wavelength and visible light. Effects of diffraction and resolution. Lens design. Defects and distortions. Magnification. Electron Duality. Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Lecture . 7: Confocal Microscopy. Optical Sectioning: adding the third dimension. Wide-field . Imaging. Point . Spread Function. Deconvolution. Microlens. Array. 18 October, . FiO. 2011. Antony Orth and Kenneth . Crozier. High Throughput Microscopy. 1. http://www.olympus.co.uk/microscopy/22_scan_R.htm#. High throughput fluorescence imaging by scanning sample under . Chelsea Aitken. Peter Aspinall. Advantages Over Light Microscopy. Resolution of light microscopy limited by Rayleigh Criterion. If two objects cannot be seen as distinct structures, then they may be considered coincident in space. Lecture 18:. High speed microscopy, Part 2. High speed microscopy, Part . 2: Spatial . light modulator microscope and other 3D sensors. Making laser scanning confocal microscopes faster. Resonant scanner confocal. II. MENA3100,OBK, . 29.01.15. We don’t read all. You don’ have to read all. 1.3 Specimen Preparation. Read it. 1.4.1 Bright-Field and Dark-Field. Definetly. 1.4.2 Phase-Contrast. Cursori. 1.4.3 Polarized-Light. 50X. magnification. 300X. magnification. 1000X. magnification. 2000X. Near sphere shape. Nonuniform size. Unsmooth surface. The sphere body consist of small strips. small . strips is . cuboid bars. pristine. Applications of Light Microscopy. Observe less detailed features of intact cell than advanced microscopy (i.e. electron and laser). Shape, presence of flagella, diagnostic stain, arrangement of cells, some large internal features. Microscopy is the technical field using microscopes to view samples and objects that can not be seen without unaided eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron and scanning probe microscopy.. Replica of van 1670 Moody Use the information in this tutorial to supplement the visuals in lab and the Chapters 1 8 and 9 in your lab manual Replica of Culpepper tripod microscope built c 1725 by Co 1Many different types of light microscopesare available each with their own strengths and limitationsA main tenant of good microscopy is to select the right tool microscope for the job at handPlease c Outline. Introduction. Optical Microscopy. Types of Optical Microscopes. Confocal Microscopy. Laser Scanning Confocal Microscopy. Examples. Optical Microscopy. Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. Amina Mustafa. Department of Molecular Biology. Virtual University of Pakistan. Invented by :. Antonie. van Leeuwenhoek. (Father of Microbiology). Microscopy. USES:. Diagnostics. Identification. Histology – Tissue analysis. Science. Learning intention. To know how microscopes have advanced over time and to be able to . compare the advantages and disadvantages of the different types of microscopes.. You will need. •  A pen and paper..