PPT-Microscopy and Magnification

1000 1000 1000 1000 mm Micrometre n m Nanometre Micrometre Millimetre 5 0005 0000005 1       1       1   3   7         05       0001 0000001 1000. Basic Introduction. Bob Ashley. 6-14-2013. Overview . Why electrons?. Wavelength and visible light. Effects of diffraction and resolution. Lens design. Defects and distortions. Magnification. Electron Duality. Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Lecture . 7: Confocal Microscopy. Optical Sectioning: adding the third dimension. Wide-field . Imaging. Point . Spread Function. Deconvolution. Features Essential for Diagnosis. Ulysses J. Balis, MD. Director, Division of Pathology Informatics. Associate Professor of Pathology. Department of Pathology. University of Michigan. ulysses@med.umich.edu. 50X. magnification. 300X. magnification. 1000X. magnification. 2000X. Near sphere shape. Nonuniform size. Unsmooth surface. The sphere body consist of small strips. small . strips is . cuboid bars. pristine. Specimen Preparation. Microscope. Basics. T. Trimpe 2005 http://sciencespot.net/. Microscopes . are . scientific instruments . designed to produce magnified visual or photographic images of small . Comparison of relative sizes of molecules, cell membrane thickness, viruses, bacteria, organelles and cells, using the appropriate SI unit.. Size . relationships. of biological and chemical. levels of organization are. Applications of Light Microscopy. Observe less detailed features of intact cell than advanced microscopy (i.e. electron and laser). Shape, presence of flagella, diagnostic stain, arrangement of cells, some large internal features. Microscopy is the technical field using microscopes to view samples and objects that can not be seen without unaided eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron and scanning probe microscopy.. Replica of van 1670 Moody Use the information in this tutorial to supplement the visuals in lab and the Chapters 1 8 and 9 in your lab manual Replica of Culpepper tripod microscope built c 1725 by Co Outline. Introduction. Optical Microscopy. Types of Optical Microscopes. Confocal Microscopy. Laser Scanning Confocal Microscopy. Examples. Optical Microscopy. Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. Amina Mustafa. Department of Molecular Biology. Virtual University of Pakistan. Invented by :. Antonie. van Leeuwenhoek. (Father of Microbiology). Microscopy. USES:. Diagnostics. Identification. Histology – Tissue analysis. AIM : T THEORY: Microscope is the most commonly used piece of apparatus in the laboratory. It produces greatly enlarged images of minute objects. LIGHT MICROSCOPE A Light Microscope can be simple or Theoretically a microscope is an array of two lenses Need of M icroscopy Living organisms, cells and their organelles which are too small to be seen with the human eye, can only be seen with the aid Science. Learning intention. To know how microscopes have advanced over time and to be able to . compare the advantages and disadvantages of the different types of microscopes.. You will need. •  A pen and paper..