PPT-16.4 – Use of Recombinant DNA Technology

Learning Objectives Understand how advances in DNA technology have benefited humans Learn how different organisms have played a part in the advancement of recombinant DNA technology What used to take a thousand years now takes weeks. PLASMID VECTORS. . . Cloning into a Plasmid. Bacteria are useful hosts.. They are easily grown. They are cheap to grow. They grow fast. They are easily manipulated in the laboratory. DNA can be inserted - transformation. Recombinant DNA Technology. T. echnique that allows DNA to be . combined. from different sources. DNA code universal. Foundation of genetic engineering. Review. What are enzymes? What function do they serve?. Objective. Students will model the process of using restriction enzymes and plasmids to form recombinant DNA.. Background Information. major tools of recombinant DNA technology are bacterial enzymes called restriction enzymes.. Will Herrick. Peyton Group Meeting. March 20, 2013. What Are Adenoviruses?. Linear . dsDNA. virus. 80-100 nm. Nonenveloped. Icosahedral structure:. 1: penton . capsomeres. 2: . hexon. . capsomeres. Recombinant . DNA technology. Methods . used to join together (recombine) different DNA segments that are not found together in nature.. This technique is used in genetic analysis to serve several applications:. Introduction. Lecture 1. Biotechnology. It implies with the use of microorganisms, plants, animals or parts of them for the production of useful compounds.. Pharmaceutical biotechnology. It is concerned as the biotechnological manufacturing of pharmaceutical products. . Recombinant . DNA technology. Methods . used to join together (recombine) different DNA segments that are not found together in nature.. This technique is used in genetic analysis to serve several applications:. DNA polymerase (copy DNA), restriction endonucleases (cut DNA), ligases (join DNA). DNA cloning – vector (plasmid, BAC), PCR. genome mapping. relative locations of genes are established by following inheritance patterns. TECHNOLOGY 1 Objectives • Recombinant DNA • Probes • Restriction map • Gene cloning • Gene library • Cloning vectors • RFLP • DNA Fingerprinting • DNA foot printing • Genomic impri great promise, and several types are being tested in. Humans. • DNA vaccines take immunization to . a new. technological. level. • . These vaccines dispense with both the whole . organism and . Biotechnology: Recombinant DNA and Cloning. Module 1 presentation B. Biotechnology: . Recombinant. DNA and Cloning. Learning intention. Describe the process of making recombinant DNA. Success criteria . Presentations. INTRODUCTION, PURPOSE, METHODS, RESULTS, CONCLUSIONS, REFERENCES.. First to isolate DNA. Fredrick . Meischer. (1868). 1950’s: What is the material of heredity?. Protein? . DNA?. Martha Chase, Alfred Hershey. Dr.V. S. Bheemareddy. Following . steps are involved in genetic engineering. .. Isolation of desired gene or DNA.. Construction of recombinant DNA. Insertion of recombinant DNA in to host cells. Selection of transformed cells or organisms. Profit. What the heck is recombinant DNA?. Recombinant DNA is what you get when you combine DNA from two different sources.. For Example:. Mouse + Human DNA. Human + Bacterial DNA. Viral + Bacteria DNA.