PPT-Last lecture summary recombinant DNA technology

DNA polymerase copy DNA restriction endonucleases cut DNA ligases join DNA DNA cloning vector plasmid BAC PCR genome mapping relative locations of genes are established by following inheritance patterns. PLASMID VECTORS. . . Cloning into a Plasmid. Bacteria are useful hosts.. They are easily grown. They are cheap to grow. They grow fast. They are easily manipulated in the laboratory. DNA can be inserted - transformation. Recombinant DNA Technology. T. echnique that allows DNA to be . combined. from different sources. DNA code universal. Foundation of genetic engineering. Review. What are enzymes? What function do they serve?. Recombinant . DNA technology. Methods . used to join together (recombine) different DNA segments that are not found together in nature.. This technique is used in genetic analysis to serve several applications:. Introduction. Lecture 1. Biotechnology. It implies with the use of microorganisms, plants, animals or parts of them for the production of useful compounds.. Pharmaceutical biotechnology. It is concerned as the biotechnological manufacturing of pharmaceutical products. . Brief Chapter Outline.  . I. Cause for Concern? Recombinant DNA Technology: Promise and Controversy. Cutting . and Joining DNA. DNA . Cloning. V. Cloning Vectors. A. . Bacterial Vectors. Dr. Brian Rymond (Instructor). &. Christen . Wanstrath. (TA). Make and break DNA (and RNA) in a variety of ways and test the consequences in the host organism (. E. coli, S. cerevisiae, C. elegans. Definition. Steps . Applications. INTRODUCTION. Recombinant DNA.  (. rDNA. ): .  . DNA.  molecules formed by laboratory methods of . genetic recombination.  (such as . molecular cloning. ) to bring together genetic material from multiple sources, creating . A deadly pathogen called the papaya . ringspot. virus (PRV) had spread throughout the islands. . It appeared poised to completely decimate the papaya plant population.. Scientists from the University of Hawaii were able to rescue the industry by creating new, genetically engineered PRV-resistant strains of papaya.. Genetic engineering involves inserting foreign genes or modifying the activity of existing genes. . This technology exploits the process of gene cloning which involves making many identical copies of a gene. . Concept and basic steps in recombinant DNA technology and gene cloning. -2-. Recombinant DNA technology is playing a vital role in improving health conditions by . developing new vaccines and pharmaceuticals. Technology AQC - 321 Dr. Mamta Singh Assistant Professor COF (BASU), Kishanganj Recombinant DNA Technology... Definition : It is a technology of joining together of DNA molecules from two different s TECHNOLOGY 1 Objectives • Recombinant DNA • Probes • Restriction map • Gene cloning • Gene library • Cloning vectors • RFLP • DNA Fingerprinting • DNA foot printing • Genomic impri Presentations. INTRODUCTION, PURPOSE, METHODS, RESULTS, CONCLUSIONS, REFERENCES.. First to isolate DNA. Fredrick . Meischer. (1868). 1950’s: What is the material of heredity?. Protein? . DNA?. Martha Chase, Alfred Hershey. Dr.V. S. Bheemareddy. Following . steps are involved in genetic engineering. .. Isolation of desired gene or DNA.. Construction of recombinant DNA. Insertion of recombinant DNA in to host cells. Selection of transformed cells or organisms.