PPT-Ion-Exchange Ultrathin Layer Chromatography (UTLC) of Biolo

HPTLC 2017 Berlin Yanhui Celia Wang and Susan V Olesik Department of Chemistry and Biochemistry Ohio State University Columbus Ohio 43016 Areas where UTLC separations of amino acids peptides and proteins may be valuable. PREPARED BY-. MD.MARUF HASSAN. Chromatography. The process or technique of separating molecules or components in a mixture . solution (gas or . liquid) . according . to the differential absorption and elution . A little history. To write with colors -- literally translated from its Greek roots . chroma. . and . graphein. , chromatography was first developed by the Russian botanist Mikhail . Tswett. in 1903 as he produced a colorful separation of plant pigments through a column of calcium carbonate, particularly the . Prepared by :. Mona . Abo. . Hasera. Chromatography. First application by M. S. . Tswett. 1903. For the separation of plant pigments. Since the components had different colors the Greek . chromatos. Chromatography. Chromatography is a technique of separation of different dissolved constituents of a mixture by absorbing them over an appropriate adsorbent material .It is a versatile method of separating different kinds of chemical mixtures .. BELARUSIAN, RUSSIAN AND KAZAKHSTAN . RAILWAYS. 2. 3. UTLC BASIC ROUTE . DOSTYK/ALTYNKOL. BREST. Distance: 5430 km. Time: . ~ . 5,5 days. Distance: ~20 000 km. Time: 28-35 days. 4. UTLC BUSINESS MODEL. (TLC). Thin layer chromatography (TLC) is an important technique for. identification. and . separation. of mixtures of organic compounds. It is useful in:. Identification of components of a mixture (using appropriate standards). Stationary phase. Mobile phase. Separation AND Characterization. Chromatography Basics. Based on different affinities for stationary and mobile phases. Silica gel: polar, water-covered surface. Compound(s). Dr. Kevin Ahern. Protein Purification. Applications of Biochemistry Knowledge. Opening Cells. Centrifugation. Fractionation. Dialysis. Applications of Biochemistry Knowledge. Separates salts from proteins. 1- Adsorption chromatography.. 2- Partition chromatography.. 3- Ion exchange chromatography.. 4- Gel filtration chromatography.. 5- Affinity chromatography.. Thin layer chromatography (TLC). is a method for . OF RAILWAYS OF . KAZAKHSTAN, RUSSIA . AND . BELARUS. 2. KAZAKHSTAN. RUSSIA. BE. LARU. S. Main. . operational. . target. . –. . high. . quality. . rail. . service. . in. . the. . Eurasian. Dr.Methak. A . ljboori. Ion Exchange Chromatography. Ion exchange chromatography (IEX) is a technique that is commonly used in . biomolecule. purification. It involves the separation of molecules on the basis of their charge. The word Chromatography is derived fro two Greek . words –. Chroma . means – colour and . graphein. . to write. . Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. Definition. Ion-exchange chromatography. (or . ion chromatography. ) is a process that allows the separation of ions and polar molecules based on the charge properties of the molecules. . 1975 by Small, Stevens and Baumann.