PPT-Whole genome sequencing and comparative transcriptome analysis of a novel seawater adapted,

Risheng Chen et al 2017 BMC Genomics Oryza sativa Cultivated rice Oryza sativa Family Poaceae grasses corn bamboo wheat SR86 New rice cultivar domesticated from a wild strain of rice first found in 1986. Mayo/UIUC Summer . C. ourse in Computational Biology. Session Outline. Genome sequencing. Schematic overview of genome assembly. (a) DNA is collected from the biological sample and sequenced. (b) The output from the sequencer consists of many billions of short, unordered DNA fragments from random positions in the genome. (c) The short fragments are compared with each other to discover how they overlap. (d) The overlap relationships are captured in a large assembly graph shown as nodes representing . Mayo/UIUC Summer . C. ourse in Computational Biology. Session Outline. Genome sequencing. Schematic overview of genome assembly. (a) DNA is collected from the biological sample and sequenced. (b) The output from the sequencer consists of many billions of short, unordered DNA fragments from random positions in the genome. (c) The short fragments are compared with each other to discover how they overlap. (d) The overlap relationships are captured in a large assembly graph shown as nodes representing . From Swab to Publication. Madison I. Dunitz. 1. , David A. Coil. 1. , Jenna M. Lang. 1. , Guillaume Jospin. 1. , Aaron E. Darling. 2. , Jonathan A. Eisen. 1. UC Davis Genome Center. 1. University of California, Davis; . Next Generation Sequencer Applications. DeNovo Sequencing. Resequencing, Comparative Genomics. Global SNP Analysis. Gene Expression Analysis. Methylation Studies. ChIP . Sequencing-transcription factors, histones, polymerases. Craig A. . Praul. Co- Director . Genomics Core Facility. Huck Institutes of the Life Sciences. Penn State University. A very short history of DNA sequencing. I started from the conviction that, if different DNA species exhibited . Next Generation Sequencing. Hanlee Ji, M.D. . ã . Stanford University. Overview. Principles of next generation . DNA sequencing. Analysis of genetic variation and research applications. How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. TGATTTTAAAAAAATATT…. Hierarchical genome shotgun HGS – Human Genome Project. “map first, sequence second. ”. clone-by-clone … cloning is performed twice (BAC, plasmid). Sequencing strategies. Whole genome shotgun WGS – Celera. Whole genome sequencing of babies REASONS FOR USING WHOLE GENOME SEQUENCING IN BABIES There are a number of possible reasons for carrying out whole genome or exome sequencing Seeking a diagnosis for a for suspected cancer Information for patients and family members Genomic Medicine Service NHS What is your genome? Your genome is the information needed to build the human body and keep it health Analysis. Jessica Holmes. High Performance Biological Computing (HPCBio). Roy J. Carver Biotechnology Center. General Outline. Getting the RNA-Seq data: from RNA -> Sequence data. Experimental and practical considerations. BIOINFORMATICA. per il CLM in BIOLOGIA EVOLUZIONISTICA. Scuola di Scienze, Università di Padova. Prof. STEFANIA BORTOLUZZI. Outline. Transcriptomics. today. RNA-. seq. features and advantages. Transcriptome. BIOINFORMATICA. per il CLM in BIOLOGIA EVOLUZIONISTICA. Scuola di Scienze, Università di Padova. Prof. STEFANIA BORTOLUZZI. Outline. Transcriptomics. today. RNA-. seq. features and advantages. Transcriptome. BIOINFORMATICA. per il CLM in BIOLOGIA EVOLUZIONISTICA. Scuola di Scienze, Università di Padova. Prof. STEFANIA BORTOLUZZI. Outline. Transcriptomics. today. RNA-. seq. features and advantages. Transcriptome.