PPT-Lecture 22 RNA – Transcription & Translation

Outline Review httphigheredmcgrawhillcomsites0072507470studentview0chapter3animationdnareplicationquiz1html RNA Transcription How mRNA is made mRNA processing Translation. Replication,. Transcription, & Translation. . DNA Replication. DNA Replication. . Before a cell can divide by . mitosis. or . meiosis. , it must first make a copy of its . chromosomes. .. The . Gene . to . Phenotype. : . The BAD2 gene. . and . fragrance in rice. DNA . sequence specifying . a . protein 200 . – 2,000,000 . nt. (. bp. ). Eukaryotic Gene Structure. Ribonucleic acid (RNA) is a key nucleic acid in transcription and translation. RNA is like DNA except that:. C483 Spring 2013. 1. RNA Polymerase. Polymerizes RNA in a 3’. 5’ direction.. Has 3’5’ . exonuclease. activity. Has 5’3’ . exonuclease. activity. Catalyzes a reaction driven by pyrophosphate hydrolysis. i. n Eukaryotes. Lecture #2. 1/23/17. References. . A few of the many insights from RNA polymerase structures. Cramer, P. (2002) . Multisubunit. RNA polymerases. . Curr. . Opin. . Struct. . Biol. Geeta. . Narlikar. ) . . January 19, 2017 – Transcription Initiation and its Regulation in Bacteria. January 23, 2017 – Transcription Initiation and its Regulation in Eukaryotes. January 26, 2016 – Chromatin 1. Topic 2.6 & 7.2. Understandings:. Transcription is the synthesis of mRNA copied from the DNA base sequences by RNA polymerase. . Gene expression is regulated by proteins that bind to specific base sequences in DNA.. A . PuhJayJay. Presentation . Daily Catalyst . 1. If there are 34% of Adenines in DNA, what is the percentage of . uracil. in RNA?. 34%. 2. .   . Replicate. the following segment of DNA:.     5’- ATCGGCTACGTTCAC  -3’. Structure. Central Dogma. Template Strand. Coding Strand. RNA Functions. RNA Building Blocks. Structure. Eukaryotic Process. RNA Polymerase. Subunits (Prokaryotes). α (2). β. β’. ω. σ. Transcription. LT: Be able to explain the process of DNA transcription.. THE BIG PICTURE!!!. DNA. RNA. protein. Transcription. Translation. Notes from reading pgs. 425-426. RNA = ribonucleic acid. Made of nucleotides. Eukaryotic Cells – cells with a nucleus and many organelles. Organisms with nucleated cells belong to the largest classification group, Domain . Eukaryota. Four Kingdoms of Organisms which are eukaryotic are animal, plants, . 1. The information content of genes is in the form of specific sequences of nucleotides along the DNA . strands. The . DNA of an organism leads to specific traits by dictating the synthesis of proteins and of RNA molecules involved in protein synthesis (. Structure of RNA. Major Classes of RNA. Transcription in Prokaryotes. Transcription in Eukaryotes. Post-transcriptional Processing of Eukaryotic mRNA. Transcriptional Regulation in Prokaryotes:. the Lac Operon as an example. Chapter . 17.1-17.3. Important Terms to Know…. 25% of students confuse these terms in . FRQs!!. Replication. Copying of DNA . prior to cell division. (DNA .  DNA). Not prior to transcription. !. A eukaryotic transcription initiation complex consists of RNA polymerase II, . five. general transcription factors (GTFs), and a 20-subunit complex called Mediator (or . Srb. /Med). The CTD of RNA polymerase II anchors Mediator to the polymerase. Mediator allows RNA polymerase II to communicate with transcriptional .