Gas chromatography Software - PowerPoint Presentation

Slide1

Gas chromatography

Software

Slide2

TRACE GC System Components

1- Pneumatic Compartment

The pneumatic compartment contains the pneumatic gas control circuits. The circuits can be completely electronic (digital pneumatics), completely analog, or a combination of analog and digital components (mixed pneumatics).2- Analytical Unit The analytical unit consists of two sub compartments:the column oventhe injector and detector compartment

Slide3

Cont.

3- Electronic Compartment

The electronic compartment consists of two sub compartments:the high-voltage compartmentthe motherboard for the detector control cards4- Display and KeypadThe display and the keypad make up the TRACE GC user interface.

Slide4

Sentinel Key

The Sentinel Key is delivered with Chrom-Card software and it should be connected to the parallel port of your computer as follows:

Remove the printer cable from the PC parallel port LPT1.Connect the Sentinel Key instead the printer cable to the PC parallel port LPT1.Re-connect the printer cable on the other end of the Sentinel Key.

Slide5

Start Chrom-Card

click twice on the icon of the relevant instrument.

Slide6

Chrom-card main menu

Slide7

Slide8

New Method Parameters

Method Title

Detection ParametersIntegration Parameters.Calculation ParametersReport ParametersReport StripchartOperator IDAnalytical Information

Slide9

2. Detection Parameters

Start Acquisition when GC starts

The start of acquisition is achieved by hardware contact between the instrument and PC. The automatic start will have effect only if this option has been enabled.Baseline compensation Through this function you can select the blank chromatogram to

be subtracted

to all chromatogram analyses.

Slide10

3. Integration Parameters

This dialog box contains all parameters used for the integration algorithm.

The integration algorithm is very simple and similar to the one used in a stand alone integrator.

Slide11

4. Calculation Parameters

This section is the analytical method part containing all the parameters for

peak calculation.Calculation This box defines the calculation method used to convert peak areas into concentrations.Calibration

Slide12

5. Report Parameters

The Report Format box permits the selection of various Report types

providing various options

Slide13

6. Report Stripchart

It permits to select and to set the

Stripchart options to be included in the report printout.

Slide14

Standard Report

A standard Report can be made consisting of all integrated peak information. The

detailed report consists of:Component namesCalculated concentrations or area %Retention timesAreasBaseline correctionResponse factors

Relative

retention time if working with relative retention time identification

The report column BC identifies the baseline correction of the peak.

The available codes are:

RS

= Resolved

FU

= Fused

TL

=

Tailing

CR = Carried

MI

= Manually integrated

^^

= Off-Scale peak

Slide15

7. Operator

ID / 8. Analytical Information

Slide16

TRACE GC Parameters

The

TRACE GC (oven, carrier, injectors and detectors) should be set according to your GC configuration.

Slide17

Oven Parameters ( Trace )

The Oven tab provides you full control of the GC oven including sub ambient options and post run programs.

Controls are for running complex temperature programs (seven levels of ramps) and controlling post run events. The Oven tab displays several control groups: Graph, Ramps, Post Run Conditions, Acquisition Time, and Oven.The graph displays a graphical representation of the oven temperature program including any post run events. An isothermal run just gives a flat line.

Slide18

Analytical Method

An

analytical method describes the treatment a sample receives during analysis. You can set parameters for:temperatures, including seven programmed changes (ramps)Pressuresflow ratesinlet typesdetector types and parameterssignal changesautosampler

parameters

timed

events before, during, and after the run

Slide19

Injector Parameters

Injectors parameters page control the Split/ Splitless injector.

Depending on your instrument configuration the window displays the appropriate fields for that inlet.For example, if you selected a right SSL inlet this tab displays.

Slide20

Carrier Parameters

The carrier gas flows through the GC column at a specific rate, measured either as a linear velocity (cm/sec) or as a flow rate (cc/min).

Common carrier gases are Helium, Hydrogen, and Nitrogen.Hydrogen offers the best chromatographic properties (optimum resolution at the highest flow rates). However, because Hydrogen is flammable, Helium is often used as an alternative for a carrier gas.

Slide21

Detector Parameters

Detectors monitor the composition of the column effluent.

This is accomplished by measuring some physical or chemical property of the eluted compounds. FID, ECD, NPD,FPD, PID, TCD and mass are typical detectors for a GC.The detector types that show up in the TRACE instrument setup window are determined by your selections in the Instrument configuration window.

Slide22

Detection parameters

The detection parameters module is the analytical method section that contains all

the parameters for data acquisition.

Slide23

Auto sampler Parameters

Slide24

Internal Standard Parameters

Slide25

Components Table

The Component

table is the analytical method section that contains all the parameters for the peak identification.The Component table offers a number of tools to enter the retention times and identification windows through the chromatogram .

Slide26

Fill Components Table

fill the Component table in an automatic way, using

the retention time of the peaks calculated from the chromatogram currently displayed.

Slide27

Sample table

The Sample Table is the analytical method section that contains all

the information about the sequence of samples to be acquired and processed.

Slide28

Concentration table

First

The user should address the standard component of interest by clicking the mouse on the concentration table grid.SecondThe cursor will automatically address the input box, ready to accept the new value, once the new value has been entered and confirmed by pressing the (17(5 key, the system updates the concentration grid.Alternatively

You can address the box by a double click, in this case the system permits

to edit

the previous content of the box

Slide29

Fill Samples Table

Fill

the sample table in an automatic way, the user supplies common information for a batch of samples and the system will automatically fill the lines with the sample name and the filename made by a root name and an increasing index.

Slide30

Sample Type

Sample amount

SA is the amount of sample material measured. This column is only available for Unk samples.Internal StandardIS is the amount of internal standard added to the analysis samples. Units of measurement must be the same as those used in measuring the sample amount

.

Dilution Factor

The XF is a scaling factor (multiplier) which may be used as a conversion factor.

This column is only available for Unk samples

Slide31

Sample being Acquired

The system permits

to view the chromatogram being acquired with the function View Sample Being Acquired.

Slide32

View Calibration Curve

According to the calculation method, the system shows the

calibration points with the peak area and the concentration.

Slide33

View Chromatogram

View Chromatogram is a tool for Chrom-Card users to view or modify the

peak integration.

Slide34

Recalculation

The Recalculation tag permits to recalculate sample batches. Chrom-Card 32

bit will perform the recalculation of the samples first-last (included) using the selected option.Sample Sequence You should supply the first-last sample to be reprocessed. Refer to Recalculate sample(s) from Sample Sequence Box.

Slide35