PDF-Bacteria Cell Walls: Gram Stain

2 Bacterial Growth1 Reproduction Binary Fission2 Factors reducing growthRefrigerationAbsence of proper nutrientsRelative lack of waterHigh salt concentrationsExtremes in pH3 Stable states Endospore. Mrs. Stewart. Medical Interventions. Central Magnet School. 2 Classes of Bacteria. Gram (-) . Thin layer of . peptidoglycan. Lipopolysaccharides. (. endotoxins. ). Stain red. Gram (+). Thick layer of . Cheng . Zhang. Thurs 1 Dec 11. MM Tutorial. Gram staining. Fixed film (heat kill bacteria etc...). Methyl violet . Lugol’s. /gram’s iodine. Decolourise with acetone. Methyl red .  . STAIN 2 (counter stain): pink-red colour. T. E. R. I. A. L. . S. T. A. I. N. I. N. G. 1. What is a Stain. A stain is a substance that adheres to a cell, giving the cell color.. The presence of color gives the cells significant contrast so are much more visible. . Clinical Bacteriology II. CLS 413. Mrs. . Deemah. . Dabbagh. CLS Department. College of Applied Medical Sciences. King Saud University. Course Content. Enterobacteriacae. Lactose Fermenters:. Escherichia coli. Physical differences & clinical . signifigance. . . Figure 4.8b. Gram- positive bacteria. Gram-negative bacteria. Figure 4.8a. Gram-Positive cell walls. Teichoic acids:. Lipoteichoic acid links to plasma membrane. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . organisms. They are used to differentiate different types of organisms or to view specific parts of organisms. Leslie Teachout. MT (ASCP), CIC. Riverton Memorial and Lander Regional Hospitals. Infection Prevention. Objectives. Discover and discuss the importance of the infection preventionist and microbiology working as a team.. Lack a nucleus. DNA is naked, a single loop not bound in a chromosome. May contain plasmids (small circular fragments of DNA). Have ribosomes, but no other organelles.. DNA present as a long circular molecule.. A.L. Noor Ameer . The reasons of staining. 1- To study their shapes . 2- . To differentiate the species of bacteria by using differential . stain.. 3- . To study the internal components of the bacterial . Differentiate bacteria into two large groups (the Gram Positive and the Gram negative).. Gram status is important in medicine; the presence or absence of a cell wall will change the bacterium's susceptibility to some antibiotics. . By. Dr. . Shnyar. Hamid Qadir. Light Microscopy. Direct examination of stained or unstained preparations by . light (bright-field) microscopy. is particularly useful for detection of bacteria, fungi, and parasites.. “We learn . . . 10% of what we read, 20% of what we hear, 30% of what we see, 50% of what we see and hear, 70% of what we discuss, 80% of what we experience, 95% of what we teach others.”. ~ William Glasser. 1 The slide is washed in water; excess water is removed using a blotting paper, dried in air and heat fixed before observing under microscope. Those bacteria that hold on to primary dye-iodine comple Faculty: Dr. Rakesh Sharda OBJECTIVES OF STAINING Improves visibiltiy by greater contrast between the organism and the background, differentiate various morphological types (by shape, size, arrangeme