PPT-6. Gas chromatography

Adv Chrom Revision 1 How does a gas chromatograph separate a mixture attraction to the column vs evaporation 2 What limitation on analytes does gas chromatography have must be volatile. Supervisor:. Anton Zavialov. By: . Elham Barazeghi. Mehrafarin Ramezani. Affinity Chromatography:. Separation of proteins by reversible interaction of proteins and specific ligand in matrix of column. Prepared by :. Mona . Abo. . Hasera. Chromatography. First application by M. S. . Tswett. 1903. For the separation of plant pigments. Since the components had different colors the Greek . chromatos. BP Schools Link. Looking for Chemicals which are hidden . BP Schools Link – . Creative Chromatography. 8. In some products the chemicals we want to measure can be hidden / masked by other bigger molecules. The next two (2) experiments introduce Gas Chromatography and Simple & Fractional Distillation. This Week. Gas Chromatography – Acetates. Pavia – p. 817 - 836. Slayden – . p. 39 - 31. . Gas Chromatograph. Gas chromatography (GC). , is a common type of . chromatography . used in . analytical . chemistry for separating and analyzing compounds that can be vaporized without decomposition. . Hazards and Controls. Flash Chromatography. Hazard . Control. Spilled solvent could contact skin. Solvent could penetrate through gloves and contact skin . Wear goggles, gloves (nitrile), and lab coat. Instrumentation. Invented by a Russian Botanist Mikhail . Tswett. in 1903 . He used chromatography . . to separate the colour . . pigments in plants from . . one another. Chromatography is a separation technique in which a mobile phase carrying a mixture moves in contact with a selectively adsorbent stationary phase. Introduction. Gas chromatography is used in many research labs, industrial labs (quality control), forensic (arson and drug analysis, toxicology, etc.), environmental labs (water, soil, air), and even in the popular TV culture (crime shows like NCIS (. Evidence. 1. Class characteristic—ink analysis, paper analysis, most handwriting analysis. 2. Individual characteristic--With document analysis, it is difficult to identify to an individual characteristic except with typewriter or printer specific marks.. Instrumental Analysis Instrumentation Paper Chromatography GC – Gas Chromatography HPLC- High Performance Liquid Chromatography IR – Infrared Spectroscopy UV-Vis Spectroscopy MS – Mass Spectrometry Lecture 7 a Chromatography I Introduction Chromatography was discovered by Russian botanist Mikhail Semyonovich Tsvett , who separated plant pigments using calcium carbonate columns (1901) Martin and Synge ( A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. Definition. Ion-exchange chromatography. (or . ion chromatography. ) is a process that allows the separation of ions and polar molecules based on the charge properties of the molecules. . 1975 by Small, Stevens and Baumann. Introduction, theory, instrumentation, . derivatization. ,. Temperature programming, advantages, disadvantages and applications. 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University.