PPT-Cell type identification in single-cell RNA-

seq data Philip Lijnzaad Overview Introduction Manual methods Existing methods SingleR CHETAH Definition of cell type Originally defined in terms of tissue function and cell morphology. DNA. RNA. PROTEIN. RNA and Transcription. RNA. R. ibo. n. ucleic . A. cid. another type of nucleic acid similar to DNA found in cells. also a polymer made of nucleotides. takes information from the nucleus to the rest of the cell. Geeta. . Narlikar. ) . . January 19, 2017 – Transcription Initiation and its Regulation in Bacteria. January 23, 2017 – Transcription Initiation and its Regulation in Eukaryotes. January 26, 2016 – Chromatin 1. PHANG LAB TALK. MAY 03, 2012. Transcriptome. The collection of . all. transcripts (RNA) presents in a given cell . ~5 % codes for proteins. The rest codes other variety of RNAs. Speculation!. Most of the genome is transcribed into some form of RNAs. Essential idea: The structure of DNA allows efficient storage of genetic information.. There is 2m of DNA in each human cell, however the most cells in the human body have a diameter of 10 . μm. . This DNA is divided in chromosomes and coiled around proteins called histones so that it can be efficiently stored in each cell's nucleus. The human genome project which has decoded the case sequence for the whole 2m of the human genome requires a data warehouse (pictured) to store the information electronically. This should give a good idea of just how efficient DNA is at storing information and why it needs to be so.. Viruses. . Presented By: Diana Marquez. Introduction. The Rhinovirus, while often synonymous with the “common cold” has also been found to be a contributing factor for the occurrence of acute respiratory conditions such as asthma and inhibit effective infection resolution in at risk individuals. Sadia. . Anjum. Virology lecture 5. 1. RNA VIRUS STRATEGIES. 2. RNA -> RNA. RNA-dependent RNA polymerase . RNA -> DNA. RNA-dependent DNA polymerase. - reverse transcriptase . Host cell DNA -> RNA. Mikel . Zaratiegui. The Paf1 protein complex in fission yeast has been found to protect protein-coding genes from inhibition by RNA-mediated silencing of transcription, by stimulating the release of nascent transcripts from DNA. . Chapter 12 DNA & RNA I.DNA A. Griffith & Transformation Frederick Griffith was trying to figure out how bacteria made people sick-how they cause a certain type of pneumonia. He isolated 2 strains(types) from mice-both cultured well,but only one caused pneumonia.The culture of the disease causing bacteria were __________________colonies while the other was rough Hailun Wang. , Pak Sham, Tiejun Tong and . Herbert Pang. Rocky 2019. December 7. th. (Saturday). 2. W. hy . scRNA. -Seq data. Propose a pathway-based analytic framework using Random Forests. Identify discriminative functional pathways related to cellular heterogeneity . Overview and Capabilities: . . High Throughput Sequencing Facility (HTSG). Functional Genomics M. ammalian Genotyping Core (MGC). Corbin Jones. Faculty Director. Piotr . Meiczkowski . Technologies . RNA is made of a long chain of nucleotides.. Differences between DNA and RNA:. 1 – the sugar on RNA is ribose instead of deoxyribose. 2 – RNA is generally single-stranded. 3 – RNA contains uracil in place of thymine. Genomics Lesson . 8_1. Hardison. 3/16/15. 1. transcriptomes. 3/16/15. 2. Transcriptome. . All the DNA that is transcribed in all the cells in an organism. Protein-coding genes. Both primary transcript and mature mRNA. .. The length of the transcription bubble is about 12 to 14 . bp. , but the length of the RNA–DNA hybrid within the bubble is only 8 to 9 . bp. . As the enzyme moves along the template, the DNA duplex reforms. nano. -switches to detect novel non-coding RNA expression . .  . Scott . A. . Tenenbaum.  . SUNY- College of Nanoscale Science and Engineering, Nanobioscience. The Basic sxRNA Concept. +. OR. Structurally interacting RNA .