PPT-Chapter 13 The Structure of RNA

RNA is made of a long chain of nucleotides Differences between DNA and RNA 1 the sugar on RNA is ribose instead of deoxyribose 2 RNA is generally singlestranded 3 RNA contains uracil in place of thymine. Yann. . Ponty. (VARNA), CNRS/. Ecole. . Polytechnique. , France. Jim Procter . (. JalView. ), University of Dundee, UK. Goals. To help your . survive. . the . RNA data jungle. .. To conceptually and . TRANSLATION . Proteins involve in many body structures . According to central dogma , the genetic information flow from DNA the RNA via transcription process then it will translate later to functional protein. . Pamela Osborn. Overview . What . are ribozymes? . Background. Structure. Hairpin, Hammerhead, and . RNase. . P. Future applications. Function . & Role of ribozymes within the body. Mechanism: Role of metals in catalysis & . La gamme de thé MORPHEE vise toute générations recherchant le sommeil paisible tant désiré et non procuré par tout types de médicaments. Essentiellement composé de feuille de morphine, ce thé vous assurera d’un rétablissement digne d’un voyage sur . Chapter 12 DNA & RNA I.DNA A. Griffith & Transformation Frederick Griffith was trying to figure out how bacteria made people sick-how they cause a certain type of pneumonia. He isolated 2 strains(types) from mice-both cultured well,but only one caused pneumonia.The culture of the disease causing bacteria were __________________colonies while the other was rough Essential idea: . The structure of DNA allows efficient storage of genetic information. . 2.6 Structure of DNA and RNA. Nature . of science: . Using . models as representation of the real world—Crick and Watson used model making to discover the structure of DNA. (1.10) . 10.1 Types of RNA. DNA vs RNA. DNA vs RNA. Translation. Micro RNA (miRNA). Types of RNA Molecules. Chapter 10: Transcription and RNA Processing. 10.2 RNA Polymerase Enzymes. Transcription Initiation. BMI/CS 776 . www.biostat.wisc.edu/bmi776/. Spring . 2018. Anthony Gitter. gitter@biostat.wisc.edu. These slides, excluding third-party material, are licensed . under . CC BY-NC 4.0. by Mark . Craven, Colin Dewey, and Anthony Gitter. Distributed Supercomputer . for. High-. Throughput. . Bioinformatic. Studies. to. . Advance. RNA Research. Michael H.W. Weber. 5. th. Pan-. Galactic. BOINC Workshop. Barcelona 2009. General . Jeremy Buhler. for GEP Alumni Workshop. RNA-Seq Pipeline for Expression Analysis. RNA Source. 37251. 20653. 9827. 5121. RNA-Seq Read Count . per Transcript. Map reads to transcripts. RNA Abundance. .. The length of the transcription bubble is about 12 to 14 . bp. , but the length of the RNA–DNA hybrid within the bubble is only 8 to 9 . bp. . As the enzyme moves along the template, the DNA duplex reforms. TRIzol. purification: precipitate out everything BUT the RNA. Two common methods for separation of RNA from DNA. Selective precipitation of RNA using Lithium Chloride (LiCl). Extraction with phenol buffered at an . Daniel Hodges, Tara Seymour, Emily Spurlin, and Rebecca Alexander. Department of Chemistry, Wake Forest University. Figure 4. . Schematic of structure reactions. 1M7 is added to flexible hydroxyl groups of the RNA and relative reactivity is measured so that structure can be deduced. Mortimer .