u sing paper chromatography Kate Andrews Lorraine Bruce Unit 1 DNA and the Genome 3 Control of gene expression Separation and identification of amino acids using paper chromatography Cells and Proteins ID: 935517
Download Presentation The PPT/PDF document "Separation and identification of amino a..." is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Slide1
Separation and identification of amino acidsusing paper chromatography
Kate Andrews
Lorraine Bruce
Slide2Unit 1 DNA and the Genome
3 Control of gene expression
Separation and identification of amino acids using paper chromatography.
Slide3Cells and Proteins
2 Proteins
Use amino acid chromatography to distinguish between amino acids.
Slide4Separation of complex mixtures between two phases:
a stationary phase
a mobile phase
Chromatography
Slide5In paper chromatography :
the stationary phase is a
uniform absorbent paper
the mobile phase is the solvent
Chromatography
Slide6Slide7Slide8Slide9SafetySolvent ethanol - highly flammableammonia (.880) - corrosive, gives off toxic fumes
Prepare in fume cupboard.
Wear goggles (BS EN 166 3) and nitrile gloves
Keep ethanol and prepared solvent away from sources of ignition
Pupils wear gloves and goggles when setting up chromatogram
Keep solvent away from sources of ignition
Keep lid off solvent and chromatography tank for as short a time as possible
Slide10Ninhydrin – harmful if swallowed. Skin, eye and respiratory irritant. NOT a carcinogen.Spray in fume cupboard; wear goggle and gloves
Some amino acids are irritant and harmful – those in protocol are low risk
Risk assessment
A risk assessment should be carried out by the technician preparing the chemicals and by the teacher supervising pupils carrying out this activity.
Slide11Slide12Slide13Slide14Paper chromatography Separation of coloured pigments e.g. plant pigments
Separation and purification of proteins from a mixture
Pharmaceutical companies use paper chromatography to analyse
different compounds in drugs
Pathology labs use paper chromatography to detect
presence/absence of chemicals in blood
Sugars, amino acids, lipids and nucleic acids can be separated and identified by spraying with appropriate reagents
Slide15Resources (Prices at December, 2015)From Scientific and Chemical Ninhydrin spray (240ml): £58.46
Chromatography paper (20x20cm) £33.75
Amino acid kit (contains 20 amino acids) £63.71
From
Timstar
Capillary tubes (
pk 100) £3.64Chromatography containers2L tall beaker (code: BEA 030 080) £6.99 each Scientific & ChemicalChromatography tank (code: CHR 100 010) £12.06 each Scientific & ChemicalChromatography tank lid (Code: CHR 100 500) £15.60 each Scientific & Chemical3L Large jar (Le Parfait Orange Top) (Code: 050009784) £10.00 for 3 Asda