PDF-Cloning Vector

pUC 1 18 Product Information Sheet V333 02 MoBiTec GmbH Germany Phone 49 551 70722 0 Fax 49 551 70722 22 E Mail infomobiteccom wwwmobiteccom Revised January 2014 1 Product p UC 1 18. Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . Recombinant . DNA technology. Methods . used to join together (recombine) different DNA segments that are not found together in nature.. This technique is used in genetic analysis to serve several applications:. Lecture #9. Lehninger. . Principles of Biochemistry. Chapter 9. DNA-based information technologies. Genomics & Proteomics. Genome. : . A genome is the complete set of genetic information in an organism.. A vehicle used to transfer genetic material such as DNA sequence from the donor organism to target cell of recipient organism(Host cell).. Also referred as carrier of DNA sequence.. Also referred as cloning vehicle or cloning DNA.. Jasmin . Sutkovic. Principles . of cloning, vectors and cloning strategies. by . Jasmin SUTJOVIC. Principles of cloning, vectors and cloning strategies. DNA CLONING. DNA cloning is a technique for reproducing DNA fragments. . Mammalian Cells. Gang Zhang, Ph. D. Research Technician II. Centre for Research in Neurodegenerative Diseases. Department of Medicine. University of Toronto, Canada. This talk based on the following publications:. Principle, Requirements, steps and applications Dr. Sudha Kumari Department of Veterinary Microbiology Bihar Veterinary College, Patna Bihar Animal Sciences Un pUC18 Product Information Sheet # V330 02 MoBiTec GmbH, Germany Phone: +49 551 70722 0 Fax: +49 551 70722 22 E - Mail: info@mobitec.com www.mobitec.com Revised January 2014 1 Product p UC18 high c DNA Cloning . DNA cloning. . is process of making several identical copy of a gene or gene fragment. . Cloning. is a . natural process. . in biology where genetically identical individuals are produced by asexually reproducing organisms such as bacteria, insects or plants. In . has four essential parts: . a method for . generating the DNA fragment for cloning; . A reaction . that inserts that fragment into the . chosen cloning vector. . a . means for introducing . that recombinant . A vector is a DNA molecule that has the abil­ity to replicate autonomously in an appropri­ate host cell and into which the gene of inter­est (a foreign genetic sequence) is integrated. When we insert a foreign genetic sequence into the vector the aim is either to obtain numer­ous copies of the gene of interest or to obtain the product of that.. Rubanraj. . Dept of Mathematics. St. Joseph’s College. Trichy. Vectors . Vector is an agent that can carry a DNA fragment into a host cell in which it is capable of replication. If it is used only for reproducing the DNA fragment, it is called a . HOD Botany. MD College. Parel. ,. Mumbai-12 . Gene Cloning. Gene cloning.  is the process in which a . gene.  of interest is located and copied (. cloned. ) out of DNA extracted from an organism. .