PPT-Last lecture summary Sequencing strategies

Hierarchical genome shotgun HGS Human Genome Project map first sequence second clonebyclone cloning is performed twice BAC plasmid Sequencing strategies Whole genome shotgun WGS Celera. Sequencing strategies. Hierarchical genome shotgun HGS – Human Genome Project. “map first, sequence second. ”. clone-by-clone … cloning is performed twice (BAC, plasmid). Sequencing strategies. . Content by . Marc . Salit. & Justin . Zook. (NIST). and. Liz Mansfield & . Zivana. . Tezak. (FDA). As delivered by Daryl Thomas. NIST Perspective on standards for genome measurement assurance. The Mitten. Essential Question. How do readers use sequencing to understand and retell the text?. 2. Topic & Grade Level. Topic. Sequencing events & building a timeline to assist students in recalling the events of a story.. Pulling It All Together. . . 2. . Objectives. Discuss how to use the tools and strategies presented in this training. Demonstrate how to appropriately apply the tools and strategies in clinical scenarios. Craig A. . Praul. Co- Director . Genomics Core Facility. Huck Institutes of the Life Sciences. Penn State University. A very short history of DNA sequencing. I started from the conviction that, if different DNA species exhibited . Gene Expression . Next-Generation . DNA Sequencing Technology. Stuart M. Brown, Ph.D. This Lecture. Learning Objectives. Illumina Sequencing Technology: . shotgun, amplicon, selected. Quality Scores. 1. . ©. copyright Bruce Blumberg 2014. All rights reserved. BioSci. D145 Lecture #4. Bruce Blumberg (blumberg@uci.edu). 4103 Nat . Sci. 2 - office hours . Tu. , . Th. 3:30-5:00 (or by appointment). How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. TGATTTTAAAAAAATATT…. mRNA. RNA-SEQ. TruSeq. . Stranded. . mRNA. RNA-SEQ. The first . step. in the . workflow. . involves. . purifying. the . poly-A. . containing. . mRNA. . molecules. . using. . poly-T. . oligo. DNA polymerase (copy DNA), restriction endonucleases (cut DNA), ligases (join DNA). DNA cloning – vector (plasmid, BAC), PCR. genome mapping. relative locations of genes are established by following inheritance patterns. DNA - the basics. Drew Berry – DNA animations. http://. www.youtube.com. /. watch?v. =. WFCvkkDSfIU&index. =4&list=PL9CBBEA5A85DBCDEF. Organisation of DNA. DNA is packed in Chromosomes . Karyotype: chromosome set of a species . DNA Cloning. 3. Introduction . into. host cell. Restriction Enzymes. PCR. Polymerase. Chain. Reaction. Antibiotic Selection. Antibiotics, such as penicillin and ampicillin, kill bacteria. Plasmids can carry genes that give host bacterium a resistance against antibiotics. PREPARING FOR TESTS BEFORE YOUR FINAL REVIEW. Know what you need to know. The type of exams (e.g., essays vs. multiple choice). The data of the exams. The scope of the exams (e.g., Are you responsible for dates? Formulas? Derivations?). : . Sequencing Strategies and Tactics. DEPT: PEACE & CONFLICT STUDIES. COURSE CODE: PCS 410. EDUCATOR: DR. DEMOLA AKINYOADE. PRESENTED BY:. AGBA POWEI VASCO . 14/SMS10/001. Table of content . Brief introduction on .